RT-PCR (Reverse Transcription - Polymerase Chain Reaction): Suatu Cara Pendeteksi Perubahan-Perubahan Ekspresi Gen pada Penyakit
The reverse transcription polymerase chain reaction (RT-PCR) is widely applied for detecting mRNA expression and cloning cDNA. This method consists of two parts: cDNA synthesis from mRNA by reverse transcription (RT) and amplification of cDNA by PCR. The RT-PCR has proved useful in amplifying very s...
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| Main Authors: | , , |
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| Format: | Book |
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Faculty of Dentistry, Universitas Indonesia,
2015-10-01T00:00:00Z.
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| Summary: | The reverse transcription polymerase chain reaction (RT-PCR) is widely applied for detecting mRNA expression and cloning cDNA. This method consists of two parts: cDNA synthesis from mRNA by reverse transcription (RT) and amplification of cDNA by PCR. The RT-PCR has proved useful in amplifying very small amount of specific mRNA to obtain cDNA clones and to detect mRNA transcripts from abnormal cells in a background of normal cells. |
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| Item Description: | 1693-9697 2355-4800 10.14693/jdi.v5i3.781 |