Histomorphometric analysis of the repair process of autogenous bone grafts fixed at rat calvaria with cyanoacrylate

• मुख्य लेखकों: Jônatas Caldeira Esteves (लेखक), Albanir Gabriel Borrasca (लेखक), Alessandra Marcondes Aranega (लेखक), Idelmo Rangel Garcia Junior (लेखक), Osvaldo Magro Filho (लेखक)
• स्वरूप: पुस्तक
• प्रकाशित: University of São Paulo, 2011-10-01T00:00:00Z.
• विषय: article
• ऑनलाइन पहुंच: Connect to this object online.
• Notes: 10.1590/S1678-77572011000500016; 1678-7757; 1678-7765
• Keywords: Tissue adhesives; Cyanoacrylates; Bone transplantation; Dentistry; RK1-715
• Source: Journal of Applied Oral Science, Vol 19, Iss 5, Pp 529-534 (2011)

OBJECTIVE: The purpose of this study was to perform histological and histometric analyses of the repair process of autogenous bone grafts fixed at rat calvaria with ethyl-cyanoacrylate adhesive. MATERIAL AND METHODS: Thirty-two rats were divided into two groups (n=16), Group I - Control and Group II - Adhesive. Osteotomies were made at the right parietal bone for graft obtainment using a 4-mm-diameter trephine drill. Then, the bone segments were fixed with the adhesive in the parietal region of the opposite side to the donor site. After 10 and 30 days, 8 animals of each group were euthanized and the calvarias were laboratorially processed for obtaining hematoxylin and eosin-stained slides for histological and histometric analyses. RESULTS: An intense inflammatory reaction was observed at the 10-day period. At 30 days, this reaction was less intense, despite the presence of adhesive at the recipient-site/graft interface. Graft incorporation to the recipient site was observed only at the control group, which maintained the highest graft size at 10 and 30 days. CONCLUSIONS: Although the fragment was stable, the presence of adhesive in Group II did not allow graft incorporation to the recipient site, determining a localized, discrete and persistent inflammatory reaction.