Screening the antigenic properties of Porphyromonas gingivalis and level of raised antibodies after antigenic injection into Balb/c Mice
Background: Different diagnostic screening tests have been developed to detect periodontal disease in the early stages. Despite these advances still, there is a need for a more practical and beneficial diagnostic test. Aim: The aim of this study was to investigate the possibility of developing such...
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| Main Authors: | , , |
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| Format: | Book |
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Wolters Kluwer Medknow Publications,
2017-01-01T00:00:00Z.
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| Summary: | Background: Different diagnostic screening tests have been developed to detect periodontal disease in the early stages. Despite these advances still, there is a need for a more practical and beneficial diagnostic test. Aim: The aim of this study was to investigate the possibility of developing such a kit based on the body immune response against Porphyromonas gingivalis. Method and Materials: This experimental study was conducted by culturing P. gingivalis and extracting its antigens. These antigens were injected into peritoneal cavity of four Balb/c mice. Finally, the pattern, type, and quantity of antibody response against P. gingivalis antigen were detected. Results of the study showed that 3.0 × 108 cells of P. gingivalis are an appropriate count for stimulating the immunization in Balb/c mice and the subsequent amount of antibody (IgG) production was 81.5 μg/ml. Result: The antigenic injections which were done in the current study could mimic the condition of periodontal disease and the raise of P. gingivalis in the body. Conclusion: The obtained data can be used in future attempts to develop practical and usable test kits against P. gingivalis. |
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| Item Description: | 0970-9290 1998-3603 10.4103/ijdr.IJDR_658_15 |