CagA status and VacA subtypes of <i>Helicobacter pylori</i> in relation to histopathologic findings in Iranian population
<b>Background/Objective:</b> The aim of this study was to detect dominant cagA/vacA genotypes of <i>Helicobacter Pylori</i> (<i>H. pylori</i>) and determine correlations between different cagA/vacA genotypes and histologic features of chronic gastritis in Iranian...
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| Main Authors: | , , , , , , , |
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| Format: | Book |
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Wolters Kluwer Medknow Publications,
2010-01-01T00:00:00Z.
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| Summary: | <b>Background/Objective:</b> The aim of this study was to detect dominant cagA/vacA genotypes of <i>Helicobacter Pylori</i> (<i>H. pylori</i>) and determine correlations between different cagA/vacA genotypes and histologic features of chronic gastritis in Iranian patients. <b> Methods:</b> Gastric biopsy was taken from 166 patients with nonulcer dyspepsia. The specimens were processed and DNA from each <i>H. pylori</i> isolate was extracted from multiple<b> </b> colony sweeps for identification<b> </b> of glmM gene. The vacA subtypes and cagA gene were tested by PCR<b> . </b> Histopathological features were recorded and graded according to partial<b> </b> Sydney system. <b> Results:</b> Of the 86 strains, 66 (76.7%) were cagA positive. The proportions of vacA gene subtypes s1, s2, m1 and m2 in the 78 strains isolated were 70.5%, 29.5%, 37.2% and 62.8%, respectively. About 83.3% of the vacA-positive strains had s1 allele. Twenty-six strains (33.3%) were positive for both cagA and m1 allele. Positive cagA status and vacA subtypes were not associated significantly with presence of neutrophil infiltration, intestinal metaplasia or <i>H. pylori </i>density. Only vacA s1 was significantly associated with more severe inflammation (P=0.02).<b> </b> The dominant genotype of <i>H. pylori</i> was vacA plus s1/m2. CagA gene positivity rate was not closely associated with severity of the disease. <b> Conclusion:</b> <i>H. pylori</i> strains showing vacA s1 genotype were associated with more severe gastritis. These findings show that vacA genotyping may have clinical relevance in Iran. |
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| Item Description: | 0377-4929 0974-5130 |