Biofilm Inhibition and Eradication Properties of Medicinal Plant Essential Oils against Methicillin-Resistant <i>Staphylococcus aureus</i> Clinical Isolates

• Main Authors: Fethi Ben Abdallah (Author), Rihab Lagha (Author), Ahmed Gaber (Author)
• Formato: Livro
• Publicado em: MDPI AG, 2020-11-01T00:00:00Z.
• Assuntos: article
• Acesso em linha: Connect to this object online.
• Notes: 10.3390/ph13110369; 1424-8247
• Keywords: methicillin-resistant <i>Staphylococcus aureus</i>; essential oils; <i>Origanum majorana</i>; <i>Rosmarinus officinalis</i>; <i>Thymus zygis</i>; antibacterial; Medicine; R; Pharmacy and materia medica; RS1-441
• Source: Pharmaceuticals, Vol 13, Iss 11, p 369 (2020)

Methicillin-resistant <i>Staphylococcus aureus</i> is a major human pathogen that poses a high risk to patients due to the development of biofilm. Biofilms, are complex biological systems difficult to treat by conventional antibiotic therapy, which contributes to >80% of humans infections. In this report, we examined the antibacterial activity of <i>Origanum majorana</i>, <i>Rosmarinus officinalis</i>, and <i>Thymus zygis</i> medicinal plant essential oils against MRSA clinical isolates using disc diffusion and MIC methods. Moreover, biofilm inhibition and eradication activities of oils were evaluated by crystal violet. Gas chromatography-mass spectrometry analysis revealed variations between oils in terms of component numbers in addition to their percentages. Antibacterial activity testing showed a strong effect of these oils against MRSA isolates, and <i>T. zygis</i> had the highest activity succeeded by <i>O. majorana</i> and <i>R. officinalis</i>. Investigated oils demonstrated high biofilm inhibition and eradication actions, with the percentage of inhibition ranging from 10.20 to 95.91%, and the percentage of eradication ranging from 12.65 to 98.01%. <i>O. majorana</i> oil had the highest biofilm inhibition and eradication activities. Accordingly, oils revealed powerful antibacterial and antibiofilm activities against MRSA isolates and could be a good alternative for antibiotics substitution.