Role of Sirt3 in Differential Sex-Related Responses to a High-Fat Diet in Mice
Metabolic homeostasis is differently regulated in males and females. Little is known about the mitochondrial Sirtuin 3 (Sirt3) protein in the context of sex-related differences in the development of metabolic dysregulation. To test our hypothesis that the role of Sirt3 in response to a high-fat diet...
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MDPI AG,
2020-02-01T00:00:00Z.
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| LEADER | 00000 am a22000003u 4500 | ||
|---|---|---|---|
| 001 | doaj_ed1c50b7d9194482b768257fea9d92c8 | ||
| 042 | |a dc | ||
| 100 | 1 | 0 | |a Marija Pinterić |e author |
| 700 | 1 | 0 | |a Iva I. Podgorski |e author |
| 700 | 1 | 0 | |a Marijana Popović Hadžija |e author |
| 700 | 1 | 0 | |a Ivana Tartaro Bujak |e author |
| 700 | 1 | 0 | |a Ana Dekanić |e author |
| 700 | 1 | 0 | |a Robert Bagarić |e author |
| 700 | 1 | 0 | |a Vladimir Farkaš |e author |
| 700 | 1 | 0 | |a Sandra Sobočanec |e author |
| 700 | 1 | 0 | |a Tihomir Balog |e author |
| 245 | 0 | 0 | |a Role of Sirt3 in Differential Sex-Related Responses to a High-Fat Diet in Mice |
| 260 | |b MDPI AG, |c 2020-02-01T00:00:00Z. | ||
| 500 | |a 2076-3921 | ||
| 500 | |a 10.3390/antiox9020174 | ||
| 520 | |a Metabolic homeostasis is differently regulated in males and females. Little is known about the mitochondrial Sirtuin 3 (Sirt3) protein in the context of sex-related differences in the development of metabolic dysregulation. To test our hypothesis that the role of Sirt3 in response to a high-fat diet (HFD) is sex-related, we measured metabolic, antioxidative, and mitochondrial parameters in the liver of Sirt3 wild-type (WT) and knockout (KO) mice of both sexes fed with a standard or HFD for ten weeks. We found that the combined effect of Sirt3 and an HFD was evident in more parameters in males (lipid content, glucose uptake, <i>pparγ</i>, <i>cyp2e1</i>, <i>cyp4a14</i>, Nrf2, MnSOD activity) than in females (protein damage and mitochondrial respiration), pointing towards a higher reliance of males on the effect of Sirt3 against HFD-induced metabolic dysregulation. The male-specific effects of an HFD also include reduced Sirt3 expression in WT and alleviated lipid accumulation and reduced glucose uptake in KO mice. In females, with a generally higher expression of genes involved in lipid homeostasis, either the HFD or Sirt3 depletion compromised mitochondrial respiration and increased protein oxidative damage. This work presents new insights into sex-related differences in the various physiological parameters with respect to nutritive excess and Sirt3. | ||
| 546 | |a EN | ||
| 690 | |a sirtuin 3 | ||
| 690 | |a high fat diet | ||
| 690 | |a sex differences | ||
| 690 | |a mice | ||
| 690 | |a oxidative stress | ||
| 690 | |a metabolic stress | ||
| 690 | |a Therapeutics. Pharmacology | ||
| 690 | |a RM1-950 | ||
| 655 | 7 | |a article |2 local | |
| 786 | 0 | |n Antioxidants, Vol 9, Iss 2, p 174 (2020) | |
| 787 | 0 | |n https://www.mdpi.com/2076-3921/9/2/174 | |
| 787 | 0 | |n https://doaj.org/toc/2076-3921 | |
| 856 | 4 | 1 | |u https://doaj.org/article/ed1c50b7d9194482b768257fea9d92c8 |z Connect to this object online. |