Piceid Octanoate Protects Retinal Cells against Oxidative Damage by Regulating the Sirtuin 1/Poly-ADP-Ribose Polymerase 1 Axis In Vitro and in rd10 Mice

• 主要な著者: Seyed Mohamadmehdi Moshtaghion (著者), Estefanía Caballano-Infantes (著者), Álvaro Plaza Reyes (著者), Lourdes Valdés-Sánchez (著者), Patricia Gallego Fernández (著者), Berta de la Cerda (著者), Maurizio S. Riga (著者), Manuel Álvarez-Dolado (著者), Pablo Peñalver (著者), Juan C. Morales (著者), Francisco J. Díaz-Corrales (著者)
• フォーマット: 図書
• 出版事項: MDPI AG, 2024-02-01T00:00:00Z.
• 主題: article
• オンライン･アクセス: Connect to this object online.

要約

Retinitis pigmentosa is a common cause of inherited blindness in adults, which in many cases is associated with an increase in the formation of reactive oxygen species (ROS) that induces DNA damage, triggering Poly-ADP-Ribose Polymerase 1 (PARP1) activation and leading to parthanatos-mediated cell death. Previous studies have shown that resveratrol (RSV) is a promising molecule that can mitigate PARP1 overactivity, but its low bioavailability is a limitation for medical use. This study examined the impact of a synthesized new acylated RSV prodrug, piceid octanoate (PIC-OCT), in the 661W cell line against H₂O₂ oxidative stress and in rd10 mice. PIC-OCT possesses a better ADME profile than RSV. In response to H₂O₂, 661W cells pretreated with PIC-OCT preserved cell viability in more than 38% of cells by significantly promoting SIRT1 nuclear translocation, preserving NAD⁺/NADH ratio, and suppressing intracellular ROS formation. These effects result from expressing antioxidant genes, maintaining mitochondrial function, reducing PARP1 nuclear expression, and preventing AIF nuclear translocation. In rd10 mice, PIC-OCT inhibited PAR-polymer formation, increased SIRT1 expression, significantly reduced TUNEL-positive cells in the retinal outer nuclear layer, preserved ERGs, and enhanced light chamber activity (all <i>p</i> values < 0.05). Our findings corroborate that PIC-OCT protects photoreceptors by modulating the SIRT1/PARP1 axis in models of retinal degeneration.

記述事項

10.3390/antiox13020201
2076-3921