Analysis of binding affinity and docking of novel fatty acid-binding protein (FABP) ligands

Fatty acid-binding proteins (FABPs) belong to a family of proteins that transports fatty acids in the cytosol and regulates cellular functions like membrane phospholipid synthesis, lipid metabolism, and mitochondrial β oxidation. In this study, we synthesized ten novel derivatives from BMS309403, a...

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Main Authors: Yasuharu Shinoda (Author), Yifei Wang (Author), Tetsunori Yamamoto (Author), Hiroyuki Miyachi (Author), Kohji Fukunaga (Author)
Format: Book
Published: Elsevier, 2020-08-01T00:00:00Z.
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042 |a dc 
100 1 0 |a Yasuharu Shinoda  |e author 
700 1 0 |a Yifei Wang  |e author 
700 1 0 |a Tetsunori Yamamoto  |e author 
700 1 0 |a Hiroyuki Miyachi  |e author 
700 1 0 |a Kohji Fukunaga  |e author 
245 0 0 |a Analysis of binding affinity and docking of novel fatty acid-binding protein (FABP) ligands 
260 |b Elsevier,   |c 2020-08-01T00:00:00Z. 
500 |a 1347-8613 
500 |a 10.1016/j.jphs.2020.05.005 
520 |a Fatty acid-binding proteins (FABPs) belong to a family of proteins that transports fatty acids in the cytosol and regulates cellular functions like membrane phospholipid synthesis, lipid metabolism, and mitochondrial β oxidation. In this study, we synthesized ten novel derivatives from BMS309403, a biphenyl azole compound specific for FABP4, and analyzed their affinity and specificity for FABP3, FABP4, and FABP5, which possess 60% of homology in amino acid sequence. Here, we used 1-anilinonaphthalene 8-sulfonic acid (ANS) displacement assay and found that Ligand 1 has highest affinity for FABP3, with comparable affinity for FABP4 and FABP5. The apparent dissociation constant of BMS309403 was identical to that of arachidonic acid and docosahexaenoic acid. Docking studies with X-ray structural data showed that these novel derivatives obtained by the substitution of phenoxyacetic acid in BMS309403 but not BMS309403 have high or moderate affinity for FABP3. We further found that substitution of a phenyl group and alkyl group caused steric hindrance between 16F, the portal loop and 115L, 117L, respectively, leading to decrease in their affinity for FABPs. In conclusion, our study provides a novel strategy for development of specific ligand for each FABP. 
546 |a EN 
690 |a Fatty acid-binding protein 
690 |a BMS309403 derivatives 
690 |a ANS displacement Assay 
690 |a Ligand docking 
690 |a Protein-ligand interaction 
690 |a Therapeutics. Pharmacology 
690 |a RM1-950 
655 7 |a article  |2 local 
786 0 |n Journal of Pharmacological Sciences, Vol 143, Iss 4, Pp 264-271 (2020) 
787 0 |n http://www.sciencedirect.com/science/article/pii/S1347861320300499 
787 0 |n https://doaj.org/toc/1347-8613 
856 4 1 |u https://doaj.org/article/ee50e216b51d44ca88804ff921a09e02  |z Connect to this object online.