Evaluating bioactive constituents and toxicological effects of aqueous extract of fermented Pentaclethra macrophylla seeds in rats

• Main Authors: Eziuche Amadike Ugbogu (Author), Chukwumaobim Daniel Nwoku (Author), Victor Chibueze Ude (Author), Okezie Emmanuel (Author)
• Format: Book
• Published: Mashhad University of Medical Sciences, 2019-12-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.
• Notes: 2228-7930; 2228-7949; 10.22038/ajp.2019.13557
• Keywords: pentaclethra macrophylla; bioactive compounds; lipid profile; hepatic enzyme; histopathology; Therapeutics. Pharmacology; RM1-950
• Source: Avicenna Journal of Phytomedicine, Vol 10, Iss 1, Pp 101-113 (2019)

<strong>Objective: </strong>This study aimed at evaluating the bioactive constituents and the toxicological profile of the aqueous fermented seed extract of <em>P. macrophylla</em>.<br /> <strong>Material and Methods: </strong>The chemical constituents of fermented <em>P. macrophylla</em> were assessed using GC-MS. For acute toxicity study, one-time doses of up to 5000 mg/kg of the extract were orally administered to male and female rats whereas 200, 400 and 800 mg/kg of the <em>P.</em><em> macrophylla</em> extract were orally administered daily for 14 days in sub-acute toxicity investigation. Biochemical, haematological and lipid profiles were assessed following standard methods.<br /> <strong>Results:</strong> Bioactive compounds such as citronellol and oxirane, tetradecyl- (hexadecylene oxide) were identified in the extract. In acute toxicity test, no death or sign of toxicity was identified. For sub-acute study, ALT decreased significantly (p<0.05) while HDL-C had dose-dependent increases. No effect (p<0.05) on haematological parameters except on platelets was found. No histopathological changes were observed.<br /> <strong>Conclusion: </strong>Our results demonstrated that the extract of fermented <em>P. macrophylla</em> caused no toxic effects in the rats at the tested doses. Therefore, they may be termed safe for consumption and therapeutic uses.