Solid-surface vitrification is an appropriate and convenient method for cryopreservation of isolated rat follicles
<p>Abstract</p> <p>Background</p> <p>Cryopreservation of isolated follicles may be a potential option to restore fertility in young women with cancer, because it can prevent the risks of cancer transmission. Several freezing protocols are available, including slow-rate...
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| Main Authors: | , , , , , , |
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| Format: | Book |
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BMC,
2010-05-01T00:00:00Z.
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| Summary: | <p>Abstract</p> <p>Background</p> <p>Cryopreservation of isolated follicles may be a potential option to restore fertility in young women with cancer, because it can prevent the risks of cancer transmission. Several freezing protocols are available, including slow-rate freezing, open-pulled straws vitrification (OPS) and solid-surface vitrification (SSV, a new freezing technique). The purpose of our study was to investigate the effects of these freezing procedures on viability, ultrastructure and developmental capacity of isolated rat follicles.</p> <p>Methods</p> <p>Isolated follicles from female Sprague-Dawley rats were randomly assigned to SSV, OPS and slow-rate freezing groups for cryopreservation. Follicle viability assessment and ultrastructural examination were performed after thawing. In order to study the developmental capacity of thawed follicles, we performed <it>in vitro </it>culture with a three-dimensional (3D) system by alginate hydrogels.</p> <p>Results</p> <p>Our results showed that the totally viable rate of follicles vitrified by SSV (64.76%) was slightly higher than that of the OPS group (62.38%) and significantly higher than that of the slow-rate freezing group (52.65%; <it>P </it>< 0.05). The ultrastructural examination revealed that morphological alterations were relatively low in the SSV group compared to the OPS and slow-rate freezing groups. After <it>in vitro </it>culture within a 3D system using alginate hydrogels, we found the highest increase (28.90 ± 2.21 μm) in follicle diameter in follicles from the SSV group. The estradiol level in the SSV group was significantly higher than those in the OPS and slow-rate freezing groups at the end of a 72-hr culture period (<it>P </it>< 0.05).</p> <p>Conclusions</p> <p>Our results suggest that the SSV method is an appropriate and convenient method for cryopreservation of isolated rat follicles compared with the conventional slow-rate freezing method and the OPS method.</p> |
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| Item Description: | 10.1186/1477-7827-8-42 1477-7827 |