Metabolism and gene polymorphisms of the folate pathway in Brazilian women with history of recurrent abortion

PURPOSE: To investigate the association between polymorphisms in genes that encode enzymes involved in folate- and vitamin B12-dependent homocysteine metabolism and recurrent spontaneous abortion (RSA).METHODS: We investigated the C677T and A1298C polymorphisms of the methylenetetrahydrofalate reduc...

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Main Authors: Wendell Vilas Boas (Author), Rozana Oliveira Gonçalves (Author), Olívia Lúcia Nunes Costa (Author), Marilda Souza Goncalves (Author)
Format: Book
Published: Federação Brasileira das Sociedades de Ginecologia e Obstetrícia, 2015-02-01T00:00:00Z.
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001 doaj_f01b5d97cbf646eeb6750fdae12f2db5
042 |a dc 
100 1 0 |a Wendell Vilas Boas  |e author 
700 1 0 |a Rozana Oliveira Gonçalves  |e author 
700 1 0 |a Olívia Lúcia Nunes Costa  |e author 
700 1 0 |a Marilda Souza Goncalves  |e author 
245 0 0 |a Metabolism and gene polymorphisms of the folate pathway in Brazilian women with history of recurrent abortion 
260 |b Federação Brasileira das Sociedades de Ginecologia e Obstetrícia,   |c 2015-02-01T00:00:00Z. 
500 |a 0100-7203 
500 |a 10.1590/SO100-720320140005223 
520 |a PURPOSE: To investigate the association between polymorphisms in genes that encode enzymes involved in folate- and vitamin B12-dependent homocysteine metabolism and recurrent spontaneous abortion (RSA).METHODS: We investigated the C677T and A1298C polymorphisms of the methylenetetrahydrofalate reductase gene (MTHFR), the A2756G polymorphism of the methionine synthase gene (MS) and the 844ins68 insertion of the cystathionine beta synthetase gene (CBS). The PCR technique followed by RFLP was used to assess the polymorphisms; the serum levels of homocysteine, vitamin B12 and folate were investigated by chemiluminescence. The EPI Info Software version 6.04 was used for statistical analysis. Parametric variables were compared by Student's t-test and nonparametric variables by the Wilcoxon rank sum test.RESULTS: The frequencies of gene polymorphisms in 89 women with a history of idiopathic recurrent miscarriage and 150 controls were 19.1 and 19.6% for the C677T, insertion, 20.8 and 26% for the A1298C insertion, 14.2 and 21.9% for the A2756G insertion, and 16.4 and 18% for the 844ins68 insertion, respectively. There were no significant differences between case and control groups in any of the gene polymorphisms investigated. However, the frequency of the 844ins68 insertion in the CBS gene was higher among women with a history of loss during the third trimester of pregnancy (p=0.003). Serum homocysteine, vitamin B12 and folate levels id not differ between the polymorphisms studied in the case and control groups. However, linear regression analysis showed a dependence of serum folate levels on the maintenance of tHcy levels.CONCLUSION: The investigated gene polymorphisms and serum homocysteine, vitamin B12 and folate levels were not associated with idiopathic recurrent miscarriage in the present study. Further investigations are needed in order to confirm the role of the CBS 844ins68 insertion in recurrent miscarriage. 
546 |a EN 
546 |a PT 
690 |a Folic acid 
690 |a Homocysteine 
690 |a Polymorphisms, genetic 
690 |a Abortion, habitual 
690 |a Vitamin B12 
690 |a Gynecology and obstetrics 
690 |a RG1-991 
655 7 |a article  |2 local 
786 0 |n Revista Brasileira de Ginecologia e Obstetrícia, Vol 37, Iss 2, Pp 71-76 (2015) 
787 0 |n http://www.scielo.br/pdf/rbgo/v37n2/0100-7203-rbgo-37-02-00071.pdf 
787 0 |n http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-72032015000200071&tlng=en 
787 0 |n https://doaj.org/toc/0100-7203 
856 4 1 |u https://doaj.org/article/f01b5d97cbf646eeb6750fdae12f2db5  |z Connect to this object online.