Low Oxygen Tension Enhances Osteogenic Potential of Bone Marrow-Derived Mesenchymal Stem Cells with Osteonecrosis-Related Functional Impairment

Objective. Glucocorticoids can affect the function of bone marrow-derived mesenchymal stem cells (BMMSCs) adversely and merit the requirement for a strategy to correct this anomaly; we assessed the effect of low oxygen (2%) on BMMSCs from rabbits with osteonecrosis. Methods. Bone marrow-derived mese...

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Main Authors: Lihong Fan (Author), Ruiyu Liu (Author), Jia Li (Author), Zhibin Shi (Author), Xiaoqian Dang (Author), Kunzheng Wang (Author)
Format: Book
Published: Hindawi Limited, 2015-01-01T00:00:00Z.
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042 |a dc 
100 1 0 |a Lihong Fan  |e author 
700 1 0 |a Ruiyu Liu  |e author 
700 1 0 |a Jia Li  |e author 
700 1 0 |a Zhibin Shi  |e author 
700 1 0 |a Xiaoqian Dang  |e author 
700 1 0 |a Kunzheng Wang  |e author 
245 0 0 |a Low Oxygen Tension Enhances Osteogenic Potential of Bone Marrow-Derived Mesenchymal Stem Cells with Osteonecrosis-Related Functional Impairment 
260 |b Hindawi Limited,   |c 2015-01-01T00:00:00Z. 
500 |a 1687-966X 
500 |a 1687-9678 
500 |a 10.1155/2015/950312 
520 |a Objective. Glucocorticoids can affect the function of bone marrow-derived mesenchymal stem cells (BMMSCs) adversely and merit the requirement for a strategy to correct this anomaly; we assessed the effect of low oxygen (2%) on BMMSCs from rabbits with osteonecrosis. Methods. Bone marrow-derived mesenchymal stem cells from normal rabbits and rabbits with osteonecrosis were divided into four groups: (1) normal-normoxia group, with normal BMMSCs cultured under 20% oxygen; (2) osteonecrosis-normoxia group, with BMMSCs from rabbits with osteonecrosis cultured under 20% oxygen; (3) osteonecrosis-low oxygen treated group, with BMMSCs from rabbits with osteonecrosis cultured under 2% oxygen; (4) normal-low oxygen treated group, with normal BMMSCs cultured under 2% oxygen. The proliferation, osteogenic, and adipogenic differentiation of MSCs and expression of stemness genes, osteogenic, and adipogenic differentiation markers were investigated. Results. Compared with BMMSCs from normal rabbits, those from osteonecrosis rabbits showed significantly reduced proliferation ability, repressed expression of stemness genes, decreased osteoblasts formation, and increased adipocytes formation, indicating an osteonecrosis-related impairment. Low oxygen (2%) treated BMMSCs from osteonecrosis rabbits showed not only increased proliferation and osteogenic potential but also decreased adipogenic potential. Conclusion. Low oxygen (2%) culture represents a novel strategy to augment BMMSC function affected by glucocorticoids and holds significance for future strategies to treat femoral head osteonecrosis. 
546 |a EN 
690 |a Internal medicine 
690 |a RC31-1245 
655 7 |a article  |2 local 
786 0 |n Stem Cells International, Vol 2015 (2015) 
787 0 |n http://dx.doi.org/10.1155/2015/950312 
787 0 |n https://doaj.org/toc/1687-966X 
787 0 |n https://doaj.org/toc/1687-9678 
856 4 1 |u https://doaj.org/article/f06b92c7247948c9b0a55cb6832d1a99  |z Connect to this object online.