Genomic Analysis of Multidrug-Resistant Hypervirulent (Hypermucoviscous) <i>Klebsiella pneumoniae</i> Strain Lacking the Hypermucoviscous Regulators (<i>rmpA</i>/<i>rmpA2</i>)

• Main Authors: Hisham N. Altayb (Author), Hana S. Elbadawi (Author), Othman Baothman (Author), Imran Kazmi (Author), Faisal A. Alzahrani (Author), Muhammad Shahid Nadeem (Author), Salman Hosawi (Author), Kamel Chaieb (Author)
• Format: Book
• Published: MDPI AG, 2022-04-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.
• Notes: 10.3390/antibiotics11050596; 2079-6382
• Keywords: antimicrobial resistance; hvKP; K2 capsule; ST14; fimbrial proteins; aerobactin; Therapeutics. Pharmacology; RM1-950
• Source: Antibiotics, Vol 11, Iss 5, p 596 (2022)

Hypervirulent <i>K. pneumoniae</i> (hvKP) strains possess distinct characteristics such as hypermucoviscosity, unique serotypes, and virulence factors associated with high pathogenicity. To better understand the genomic characteristics and virulence profile of the isolated hvKP strain, genomic data were compared to the genomes of the hypervirulent and typical <i>K. pneumoniae</i> strains. The <i>K. pneumoniae</i> strain was isolated from a patient with a recurrent urinary tract infection, and then the string test was used for the detection of the hypermucoviscosity phenotype. Whole-genome sequencing was conducted using Illumina, and bioinformatics analysis was performed for the prediction of the isolate resistome, virulome, and phylogenetic analysis. The isolate was identified as hypermucoviscous, type 2 (K2) capsular polysaccharide, ST14, and multidrug-resistant (MDR), showing resistance to ciprofloxacin, ceftazidime, cefotaxime, trimethoprim-sulfamethoxazole, cephalexin, and nitrofurantoin. The isolate possessed four antimicrobial resistance plasmids (<i>p</i>KPN3-307_type B, <i>p</i>ECW602, <i>p</i>MDR, and <i>p</i>3K157) that carried antimicrobial resistance genes (ARGs) (<i>bla</i>_OXA-1,<i>bla</i>_CTX-M-15, <i>sul2</i>, <i>APH(3")-Ib</i>, <i>APH(6)-Id</i>, and <i>AAC(6')-Ib-cr6</i>). Moreover, two chromosomally mediated ARGs (<i>fosA6</i> and <i>SHV-28)</i> were identified. Virulome prediction revealed the presence of 19 fimbrial proteins, one aerobactin (<i>iutA</i>) and two salmochelin (<i>iroE</i> and <i>iroN</i>). Four secretion systems (T6SS-I (13), T6SS-II (9), T6SS-III (12), and Sci-I T6SS (1)) were identified. Interestingly, the isolate lacked the known hypermucoviscous regulators (<i>rmpA/rmpA2</i>) but showed the presence of other <i>RcsAB</i> capsule regulators (<i>rcsA</i> and <i>rcsB</i>). This study documented the presence of a rare MDR hvKP with hypermucoviscous regulators and lacking the common capsule regulators, which needs more focus to highlight their epidemiological role.