A simple broth-disk method to determine the minimum inhibitory concentration of ceftriaxone on <i> <i>Salmonella</i> enterica</i> serovar typhi and paratyphi

• Main Authors: Bhat K (Author), Tripathy Amruta (Author), Rajagopal Rajasree (Author), Ramachandran Sreeja (Author)
• Format: Book
• Published: Wolters Kluwer Medknow Publications, 2009-04-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Summary

<b>Background and Purpose: </b> Resistance to fluoroquinolones and cephalosporins is a major problem with <i> Salmonella enterica </i> serovar Typhi and Paratyphi. An accurate determination of antibiotic susceptibility requires tests for minimum inhibitory concentration (MIC) of antibiotics. We describe a simple broth-disk method to determine the MIC of ceftriaxone on <i> S. typhi</i> and <i> S. paratyphi</i> . <b> Materials and Methods: </b> Sixteen strains of <i> S. typhi</i> and two strains each of <i> S. paratyphi A</i> and <i> S. paratyphi</i> <i>B</i> were used in the study. The MIC of ceftriaxone was determined using the simple broth-disk method and the conventional broth macrodilution method and the results were compared. <b> Results: </b> All salmonella strains were susceptible to ceftriaxone. The results of the broth-disk and the conventional broth macrodilution method were similar. <b> Conclusion: </b> The broth-disk method is a simple, reliable and cost-effective method to determine the MIC of ceftriaxone on <i> S. typhi</i> and <i>S. paratyphi A</i>.

Item Description

0377-4929