Green Synthesis of Silver Nanoparticle from <i>Anadenanthera colubrina</i> Extract and Its Antimicrobial Action against ESKAPEE Group Bacteria

• Main Authors: Anastácia Nikolaos Deonas (Author), Lucas Marcelino dos Santos Souza (Author), Gabriel Jonathan Sousa Andrade (Author), Jennifer Germiniani-Cardozo (Author), Débora Dahmer (Author), Admilton Gonçalves de Oliveira (Author), Gerson Nakazato (Author), José Marcelo Domingues Torezan (Author), Renata Katsuko Takayama Kobayashi (Author)
• Format: Book
• Published: MDPI AG, 2024-08-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Summary

Given the urgent need for novel methods to control the spread of multidrug-resistant microorganisms, this study presents a green synthesis approach to produce silver nanoparticles (AgNPs) using the bark extract from <i>Anadenanthera colubrina</i> (Vell.) Brenan var. colubrina. The methodology included obtaining the extract and characterizing the AgNPs, which revealed antimicrobial activity against MDR bacteria. <i>A. colubrina</i> species is valued in indigenous and traditional medicine for its medicinal properties. Herein, it was employed to synthesize AgNPs with effective antibacterial activity (MIC = 19.53-78.12 μM) against clinical isolates from the ESKAPEE group, known for causing high hospitalization costs and mortality rates. Despite its complexity, AgNP synthesis is an affordable method with minimal environmental impacts and risks. Plant-synthesized AgNPs possess unique characteristics that affect their biological activity and cytotoxicity. In this work, <i>A. colubrina</i> bark extract resulted in the synthesis of nanoparticles measuring 75.62 nm in diameter, with a polydispersity index of 0.17 and an average zeta potential of −29 mV, as well as low toxicity for human erythrocytes, with a CC₅₀ value in the range of 961 μM. This synthesis underscores its innovative potential owing to its low toxicity, suggesting applicability across several areas and paving the way for future research.

Item Description

10.3390/antibiotics13080777
2079-6382