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Anti-Listerial Activity of Bacteriocin-like Inhibitory Substance Produced by <i>Enterococcus lactis</i> LBM BT2 Using Alternative Medium with Sugarcane Molasses

<i>Listeria monocytogenes</i> is a foodborne pathogen that contaminates food-processing environments and persists within biofilms on equipment, thus reaching final products by cross-contamination. With the growing demand for clean-label products, the search for natural antimicrobials as...

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Main Authors: Taciana Freire de Oliveira (Author), Taís Mayumi Kuniyoshi (Author), Elionio Galvão Frota (Author), Sebastián Bermúdez-Puga (Author), Letícia Naomy Sakaue (Author), Luara Lucena Cassiano (Author), Leonardo Tachibana (Author), Rosane Aparecida Moniz Piccoli (Author), Attilio Converti (Author), Ricardo Pinheiro de Souza Oliveira (Author)
Format: Book
Published: MDPI AG, 2024-02-01T00:00:00Z.
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Summary:<i>Listeria monocytogenes</i> is a foodborne pathogen that contaminates food-processing environments and persists within biofilms on equipment, thus reaching final products by cross-contamination. With the growing demand for clean-label products, the search for natural antimicrobials as biopreservants, such as bacteriocins, has shown promising potential. In this context, this study aimed to evaluate the anti-listerial action of bacteriocins produced by <i>Enterococcus lactis</i> LBM BT2 in an alternative medium containing sugarcane molasses (SCM). Molecular analyses were carried out to characterize the strain, including the presence of bacteriocin-related genes. In the kinetic study on SCM medium <i>E. lactis,</i> LBM BT2 showed biomass and bacteriocin productions similar to those observed on a sucrose-based medium (control), highlighting the potential of the sugarcane molasses as a low-cost substrate. Stability tests revealed that the molecule remained active in wide ranges of pH (4-10) and temperature (60-100 °C). Furthermore, the proteolytic treatment reduced the biomolecule's antimicrobial activity, highlighting its proteinaceous nature. After primary purification by salting out and tangential flow filtration, the bacteriocin-like inhibitory substance (BLIS) showed bacteriostatic activity on suspended <i>L. monocytogenes</i> cells and against biofilm formation at a concentration of 0.625 mg/mL. These results demonstrate the potential of the produced BLIS as a biopreservative in the food industry.
Item Description:10.3390/antibiotics13030210
2079-6382

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