Development and validation of a normal-phase HPTLC method for the simultaneous analysis of Lamivudine and Zidovudine in fixed-dose combination tablets
Simultaneous quantification of Lamivudine and Zidovudine in tablets by HPTLC method was developed and validated. The chromatograms were developed using a mobile phase of toluene:ethyl acetate:methanol (4:4:2, v/v/v) on pre-coated plate of silica gel GF aluminum TLC plate and quantified by densitomet...
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Elsevier,
2012-04-01T00:00:00Z.
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| 001 | doaj_f4da1e7e4be64315b59e87b4d2e7e5f4 | ||
| 042 | |a dc | ||
| 100 | 1 | 0 | |a Palani Venkatesh |e author |
| 700 | 1 | 0 | |a Mahesh Daggumati |e author |
| 245 | 0 | 0 | |a Development and validation of a normal-phase HPTLC method for the simultaneous analysis of Lamivudine and Zidovudine in fixed-dose combination tablets |
| 260 | |b Elsevier, |c 2012-04-01T00:00:00Z. | ||
| 500 | |a 2095-1779 | ||
| 500 | |a 10.1016/j.jpha.2011.11.002 | ||
| 520 | |a Simultaneous quantification of Lamivudine and Zidovudine in tablets by HPTLC method was developed and validated. The chromatograms were developed using a mobile phase of toluene:ethyl acetate:methanol (4:4:2, v/v/v) on pre-coated plate of silica gel GF aluminum TLC plate and quantified by densitometric absorbance mode at 276 nm. The Rf values were 0.41±0.03 and 0.60±0.04 for Lamivudine and Zidovudine, respectively. The linearity of the method was found to be within the concentration range of 50â250 ng/spot for Lamivudine and for Zidovudine, it was 100â500 ng/spot. The lower limits of detection and quantification were 2.23 ng/spot and 7.90 ng/spot for Lamivudine and 2.90 ng/spot and 8.85 ng/spot for Zidovudine. The method was also validated for precision, specificity and recovery. This developed method was used to analyze fixed-dose tablets (Duovir, Cipla Ltd) samples of Lamivudine and Zidovudine. Keywords: Normal-phase HPTLC, Lamivudine, Zidovudine, Methanol | ||
| 546 | |a EN | ||
| 690 | |a Therapeutics. Pharmacology | ||
| 690 | |a RM1-950 | ||
| 655 | 7 | |a article |2 local | |
| 786 | 0 | |n Journal of Pharmaceutical Analysis, Vol 2, Iss 2, Pp 152-155 (2012) | |
| 787 | 0 | |n http://www.sciencedirect.com/science/article/pii/S2095177911000487 | |
| 787 | 0 | |n https://doaj.org/toc/2095-1779 | |
| 856 | 4 | 1 | |u https://doaj.org/article/f4da1e7e4be64315b59e87b4d2e7e5f4 |z Connect to this object online. |