Development of a sensitive competitive enzyme-linked immunosorbent assay for serodiagnosis of Burkholderia mallei, a Tier 1 select agent.

• Main Authors: Ulrich Wernery (Author), Elaine Chan (Author), Rekha Raghavan (Author), Jade L L Teng (Author), Ginu Syriac (Author), Sing-Yung Siu (Author), Marina Joseph (Author), Man-Lung Yeung (Author), Lilong Jia (Author), Jian-Piao Cai (Author), Tsz-Ho Chiu (Author), Susanna K P Lau (Author), Patrick C Y Woo (Author)
• Format: Book
• Published: Public Library of Science (PLoS), 2021-12-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Glanders is a highly contagious and potentially serious disease caused by Burkholderia mallei, a Tier 1 select agent. In this study, we raised a monoclonal antibody (mAb) against the lipopolysaccharide (LPS) of B. mallei and developed a competitive enzyme-linked immunosorbent assay (cELISA) for B. m...