Anticataract activity of ethanolic extract from Hippobroma longiflora (L.) G.Don leaves: Ex vivo investigation

• Main Authors: Eva Imelda (Author), Khairan Khairan (Author), Rodiah R. Lubis (Author), Pati Kemala (Author), Utari Zulfiani (Author), Suci Rahayu (Author), Ghazi M. Idroes (Author), Sarra M. Adev (Author), Nur B. Maulydia (Author), Rinaldi Idroes (Author)
• Format: Book
• Published: GarVal Editorial Ltda., 2023-09-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Summary

Context: In Aceh, Hippobroma longiflora, known as kitolodby the locals, is believed to possess medicinal benefits such as anticataract. Aims: To investigate the anticataract activity of ethanolic extract from H. longiflora leaves ex vivo. Methods: The dried fine powder of H. longiflora was macerated using ethanol at room temperature, and the concentrated extract was prepared in concentrations ranging from 100 to 300 μg/mL. The cataract model was established by using goat lens induced with H2O2 and later exposed to the extract for treatment. Anti-cataract activity was assessed by observing the change in lens opacity. Further, malondialdehyde levels and activities of endogenous antioxidants (superoxide dismutase, glutathione peroxidase, and catalase). Results: The apparent cataract opacity reduced after the exposure of the extract was observed macroscopically. The cataract eyes experienced a significant increase (p<0.05) in malondialdehyde level and a significant reduction (p<0.05) in the activities of superoxide dismutase, glutathione peroxidase, and catalase. The pathologic conditions could be reversed by introducing the extract with a concentration as low as 100 μg/mL (p<0.05). Conclusions: The ethanolic extract of H. longiflora leaf has a potential anticataract effect by ameliorating oxidative stress-related biocompounds.

Item Description

10.56499/jppres23.1691_11.5.833
0719-4250