EXPRESSION OF THE TUMOR-ASSOCIATED PROTEINS IN THE OVARIAN CANCER

• প্রধান লেখক: T. S. Bobrova (Author), K. I. Zhordania (Author), Yu. V. Chuyev (Author)
• বিন্যাস: গ্রন্থ
• প্রকাশিত: ABV-press, 2014-09-01T00:00:00Z.
• বিষয়গুলি: article
• অনলাইন ব্যবহার করুন: Connect to this object online.
• Notes: 1994-4098; 1999-8627; 10.17650/1994-4098-2008-0-1-65-72
• Keywords: Gynecology and obstetrics; RG1-991
• Source: Опухоли женской репродуктивной системы, Vol 0, Iss 1, Pp 65-72 (2014)

With the help of monoclonal antibodies (MCA-1, MCA-3, G1 class immunoglobulins) and polyspecific antiserum (As) by means of immunoblotting (IB) the increase of the expression of the tumor-associated proteins (TAP) in the samples of the tumor tissues (in 94, 55 and 38% respectively), metastatic lymph nodes - LN(m+) and sera of the patients with ovarian cancer (OC) (in 82, 77 and ~100% of the cases, respectively), was detected. The important feature of As was the reactivity with the samples of the LN (m+) on the numerous proteins (>5-10) in 78% of the patients. The statistical analysis of the data using Student t-criterion revealed the significant differences (MCA-1, MCA-3) in the expression of the markers in the tumor tissues and blood sera of the oncological patients compar- ing with the samples of the adjacent tissues, blood sera of the patients with the other tumor localization and healthy donors (p<0,05). The further research using a larger amount of the clinical material and development of the more precise quantitative immunoenzyme analysis to measure TAP, is needed. It is possible that detection of TAP using MCA-1 and As may serve a basis for the creation of the test-system for the immunodiagnostics of the OC and determination of the stage of the tumor process.