Citotoxicity of different amounts of sodium hypochlorite on human cultured osteoblasts

• Glavni autori: Tatiana Kelly da Silva Fidalgo (Autor), Roberta Barcelos (Autor), Débora Barreiros Petrópolis (Autor), Bruno Rocha Azevedo (Autor), Laura Guimarães Primo (Autor), Fernando Costa e Ssilva Filho (Autor)
• Format: Knjiga
• Izdano: Faculdade São Leopoldo Mandic, 2009-10-01T00:00:00Z.
• Teme: article
• Online pristup: Connect to this object online.
• Notes: 0103-6971; 1981-8637
• Keywords: sodium hypochlorite; root canal irrigants; osteoblas; toxicity; hipoclorito de sódio; irrigantes do canal radicular; osteoblastos; toxicidade; Dentistry; RK1-715
• Source: RGO: Revista Gaúcha de Odontologia, Vol 57, Iss 3, Pp 317-321 (2009)

Objective: To evaluate the cytotoxic effect of different amounts of sodium hypochlorite, on a culture of human osteoblastos (HOB) cells.Method: Cultures of human osteoblasts (HOB) in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% of bovine fetal serum were incubated in sodium hypochlorite (concentrations of 0.5; 1.0; 2.5 and 5.25%) for thirty seconds. The control group was represented by cells incubated in phosphate buffered saline (PBS). Cell viability was assessed by means of 0.4% trypan blue dye exclusion test, in triplicate. During the incubation period, images were recorded through an inverted optic microscope to evaluate the cellular morphology. Results: In the control group 98.7% of viable cells were verified, without morphology alterations, while no viable cells were observed in the experimental groups. The kinetics of cytotoxity was concentration-dependent. Conclusion: It was concluded that there was a cytotoxic effect on cultures of human osteoblasts incubated for thirty seconds in sodium hypochlorite in all concentrations (0.5; 1.0; 2.5 and 5.25%)