Prox1 Is a Marker for AII Amacrine Cells in the Mouse Retina

The transcription factor Prox1 is expressed in multiple cells in the retina during eye development. This study has focused on neuronal Prox1 expression in the inner nuclear layer (INL) of the adult mouse retina. Prox1 immunostaining was evaluated in vertical retinal sections and whole mount preparat...

Full description

Saved in:
Bibliographic Details
Main Authors: Luis Pérez de Sevilla Müller (Author), Shaghauyegh S. Azar (Author), Janira de los Santos (Author), Nicholas C. Brecha (Author)
Format: Book
Published: Frontiers Media S.A., 2017-05-01T00:00:00Z.
Subjects:
Online Access:Connect to this object online.
Tags: Add Tag
No Tags, Be the first to tag this record!

MARC

LEADER 00000 am a22000003u 4500
001 doaj_f7f1e2bc8c2c43ae9dd80528eff3b0b7
042 |a dc 
100 1 0 |a Luis Pérez de Sevilla Müller  |e author 
700 1 0 |a Shaghauyegh S. Azar  |e author 
700 1 0 |a Janira de los Santos  |e author 
700 1 0 |a Nicholas C. Brecha  |e author 
700 1 0 |a Nicholas C. Brecha  |e author 
700 1 0 |a Nicholas C. Brecha  |e author 
700 1 0 |a Nicholas C. Brecha  |e author 
245 0 0 |a Prox1 Is a Marker for AII Amacrine Cells in the Mouse Retina 
260 |b Frontiers Media S.A.,   |c 2017-05-01T00:00:00Z. 
500 |a 1662-5129 
500 |a 10.3389/fnana.2017.00039 
520 |a The transcription factor Prox1 is expressed in multiple cells in the retina during eye development. This study has focused on neuronal Prox1 expression in the inner nuclear layer (INL) of the adult mouse retina. Prox1 immunostaining was evaluated in vertical retinal sections and whole mount preparations using a specific antibody directed to the C-terminus of Prox1. Strong immunostaining was observed in numerous amacrine cell bodies and in all horizontal cell bodies in the proximal and distal INL, respectively. Some bipolar cells were also weakly immunostained. Prox1-immunoreactive amacrine cells expressed glycine, and they formed 35 ± 3% of all glycinergic amacrine cells. Intracellular Neurobiotin injections into AII amacrine cells showed that all gap junction-coupled AII amacrine cells express Prox1, and no other Prox1-immunostained amacrine cells were in the immediate area surrounding the injected AII amacrine cell. Prox1-immunoreactive amacrine cell bodies were distributed across the retina, with their highest density (3887 ± 160 cells/mm2) in the central retina, 0.5 mm from the optic nerve head, and their lowest density (3133 ± 350 cells/mm2) in the mid-peripheral retina, 2 mm from the optic nerve head. Prox1-immunoreactive amacrine cell bodies comprised ~9.8% of the total amacrine cell population, and they formed a non-random mosaic with a regularity index (RI) of 3.4, similar to AII amacrine cells in the retinas of other mammals. Together, these findings indicate that AII amacrine cells are the predominant and likely only amacrine cell type strongly expressing Prox1 in the adult mouse retina, and establish Prox1 as a marker of AII amacrine cells. 
546 |a EN 
690 |a Prox1 
690 |a transcription factor 
690 |a glycine 
690 |a AII amacrine cells 
690 |a mouse retina 
690 |a vision 
690 |a Neurosciences. Biological psychiatry. Neuropsychiatry 
690 |a RC321-571 
690 |a Human anatomy 
690 |a QM1-695 
655 7 |a article  |2 local 
786 0 |n Frontiers in Neuroanatomy, Vol 11 (2017) 
787 0 |n http://journal.frontiersin.org/article/10.3389/fnana.2017.00039/full 
787 0 |n https://doaj.org/toc/1662-5129 
856 4 1 |u https://doaj.org/article/f7f1e2bc8c2c43ae9dd80528eff3b0b7  |z Connect to this object online.