Determination of 20(S)-protopanaxadiol in human plasma by HPLC-MS/MS: application to a pharmacokinetic study
A rapid, specific and sensitive HPLC-MS/MS method was developed and validated for the determination of 20(S)-protopanaxadiol (PPD) in human plasma. PPD and the internal standard PD were extracted from plasma by liquid-liquid extraction with cyclohexane-methylene dichloride (2:1, v/v). The separation...
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| Main Authors: | , , , , , , , , |
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| Format: | Book |
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Elsevier,
2013-12-01T00:00:00Z.
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| Summary: | A rapid, specific and sensitive HPLC-MS/MS method was developed and validated for the determination of 20(S)-protopanaxadiol (PPD) in human plasma. PPD and the internal standard PD were extracted from plasma by liquid-liquid extraction with cyclohexane-methylene dichloride (2:1, v/v). The separation was performed on a HyPURIYTY C18 column using methanol-5 mM ammonium formate (90:10, v/v) as mobile phase at a flow rate of 0.35 mL/min. Mass spectrometric detection was carried out by electrospray ionization (ESI) in the positive ion mode using multiple reaction monitoring (MRM). The monitored transitions were m/z 425.4→217.2 for PPD and at m/z 461.4→425.5 for PD. The method was linear over the range 0.512-100 ng/mL with a lower limit of quantification (LLOQ) of 0.512 ng/mL. The mean extraction recovery of PPD was greater than 78.2% and no significant matrix effect was detected. The intra- and inter-day precisions were less than 10% and the biases below 4% for PPD. The validated method was applied to a three-level single-dose clinical pharmacokinetics study of 12 healthy Chinese volunteers and the main pharmacokinetic parameters of PPD were obtained. |
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| Item Description: | 2211-3835 2211-3843 10.1016/j.apsb.2013.10.002 |