The decrease of fibroblasts and fibroblast growth factor-2 expressions as a result of X-ray irradiation on the tooth extraction socket in Rattus novergicus

• Main Authors: Fatma Yasmin Mahdani (Author), Intan Nirwana (Author), Jenny Sunariani (Author)
• Format: Book
• Published: Universitas Airlangga, 2015-06-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Summary

<span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Background:</strong><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em> Wound healing involves cellular, molecular, physiological, and biochemical processes as responses to tissue damage. </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>For instance, when a failure during tooth extraction occurs, radiographic examination, X-rays, is required. X-rays as an enforcer </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>diagnosis can damage DNA chain, resulting in cell death and inhibition of wound healing process. </em><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Purpose: </strong><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>This research aims to </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>analyze fibroblasts cell number and fibroblast growth factor-2 (FGF-2) expressions during wound healing process after tooth extraction </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>as a result of X-ray irradiation. </em><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Methods:</strong><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em> There were three research groups, each consisting of ten rats. Incisor tooth extraction was </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>performed on the left lower jaw, and then X-ray examination was conducted at certain irradiation doses, namely 0 mSv, 0.08 mSv, </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>and 0.16 mSv. Those animals were sacrificed on day 3, and on day 7 after the extraction, histopathology and immunohistochemistry </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>examinations were conducted to determine fibroblast cell number and FGF-2 expressions. Data obtained were then analyzed by oneway ANOVA and Tukey HSD tests. </em><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Results: </strong><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>The number of fibroblasts decreased significantly in the group with the irradiation dose </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>of 0.16 mSv applied on day 7 after the extraction (p <0.05). Similarly, the number of FGF-2 expressions decreased significantly in </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>the group with the irradiation dose of 0.16 mSv applied on days 3 and 7 after the extraction (p <0.05). </em><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Conclusion: </strong><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>X-ray irradiation </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>at a dose of 0.16 mSv can inhibit the healing process of tooth extraction wound due to the decreasing of fibroblasts cell number and </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>FGF-2 expressions.</em></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span>

Item Description

1978-3728
2442-9740
10.20473/j.djmkg.v48.i2.p94-99