Transcription Factor EepR Is Required for <i>Serratia marcescens</i> Host Proinflammatory Response by Corneal Epithelial Cells
Relatively little is known about how the corneal epithelium responds to vision-threatening bacteria from the Enterobacterales order. This study investigates the impact of <i>Serratia marcescens</i> on corneal epithelial cell host responses. We also investigate the role of a bacterial tra...
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| Main Authors: | , , |
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| Format: | Book |
| Published: |
MDPI AG,
2021-06-01T00:00:00Z.
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| Online Access: | Connect to this object online. |
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| Summary: | Relatively little is known about how the corneal epithelium responds to vision-threatening bacteria from the Enterobacterales order. This study investigates the impact of <i>Serratia marcescens</i> on corneal epithelial cell host responses. We also investigate the role of a bacterial transcription factor EepR, which is a positive regulator of <i>S. marcescens</i> secretion of cytotoxic proteases and a hemolytic surfactant. We treated transcriptomic and metabolomic analysis of human corneal limbal epithelial cells with wild-type bacterial secretomes. Our results show increased expression of proinflammatory and lipid signaling molecules, while this is greatly altered in <i>eepR</i> mutant-treated corneal cells. Together, these data support the model that the <i>S. marcescens</i> transcription factor EepR is a key regulator of host-pathogen interactions, and is necessary to induce proinflammatory chemokines, cytokines, and lipids. |
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| Item Description: | 10.3390/antibiotics10070770 2079-6382 |