Phenotypic and molecular characterization of clinically isolated <i><i>Escherichia</i> coli</i>
All diarrheagenic <i>Escherichia coli</i> carry at least one virulence-related property. Stool samples from 244 patients having acute or persistent diarrhea received after the exclusion of routine enteropathogens were investigated. Purely or predominantly isolated <i>E. coli</i&...
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| Main Authors: | , , , |
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| Format: | Book |
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Wolters Kluwer Medknow Publications,
2010-07-01T00:00:00Z.
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| Summary: | All diarrheagenic <i>Escherichia coli</i> carry at least one virulence-related property. Stool samples from 244 patients having acute or persistent diarrhea received after the exclusion of routine enteropathogens were investigated. Purely or predominantly isolated <i>E. coli</i> (n = 100) were subjected to serotyping, of which only 25 were typable. They belonged to 14 different O-serogroups comprising 5 O153, 4 O102, 3 O25, 2 each of O130 and O169, and 1 each of O1, O8, O15, O37, O86, O101, O127, O143, and O160. The typable <i>E. coli</i> isolates along with 5 other untypable isolates were investigated for molecular markers, such as intimin (<i>eae</i>), enterohemolysin (<i>EhlyA</i>), a-hemolysin, heat-labile enterotoxins (<i>LT</i>), heat-stable enterotoxins (<i>STa</i>), verotoxins (<i>VT1</i> and <i>VT2</i>), invasivity (<i>ial</i>), enteroaggregative <i>E. coli</i> (EAEC) gene (<i>EAGG</i>), and enterotoxin (<i>EAST</i>). Two of the isolates (O153 and O86) were positive for enterohemolysin phenotypically and 5 for β-hemolysin both phenotypically and genotypically. Interestingly, 16.6% of the randomly isolated <i>E. coli</i> were O153, a serogroup common in cattle, and 10% belonged to EAEC pathotype of which two-thirds had the <i>EAST</i> gene, which is quite frequent in these strains. Additionally, there was one strain (O153) that was positive for <i>EAST</i> only. Between the two 0130:H6 strains isolated, one belonged to EAEC serogroup. None of the <i>E. coli</i> isolated were positive for verotoxins, <i>eae</i>, <i>LT1</i>, <i>STa</i>, and <i>ial</i>. Data obtained emphasize the need for additional research into the role of <i>eae</i> gene and other putative factors affecting the virulence of diarrheagenic <i>E. coli</i> in India. |
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| Item Description: | 0377-4929 0974-5130 |