Application of Bacteriophages to Control <i>Vibrio alginolyticus</i> Contamination in Oyster (<i>Saccostrea glomerata</i>) Larvae

• Main Authors: Tuan Son Le (Author), Paul C. Southgate (Author), Wayne O'Connor (Author), Sang V. Vu (Author), D. İpek Kurtböke (Author)
• Format: Book
• Published: MDPI AG, 2020-07-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Summary

Mortalities of bivalve larvae and spat linked with <i>Vibrio</i> spp. infection have been described in hatcheries since 1959, causing potential development of resistant bacteria. A reliable and sustainable solution to this problem is yet to be developed. Potential treatment of bacterial infection with bacteriophages is gaining interest in aquaculture as a more sustainable option for managing <i>Vibrio</i> spp. infection. This study assessed the effectiveness of bacteriophages (Φ-5, Φ-6, and Φ-7) against pathogenic <i>Vibrio</i> isolates (USC-26004 and USC-26005). These phage isolates were found to belong to the <i>Myoviridae</i> viral family. A total of 212 ORFs of Φ-5 were identified and annotated. The genome of this phage contained putative thymidine kinase and lysin enzyme. During infections with phages, the OD values of the isolates USC-26005 and USC-26004 remained stable at a much lower reading compared to the control after 9 h of incubation. Mortality rate of oyster (<i>Saccostrea glomerata</i>) larvae was 28.2 ± 3.5% in the bacteriophage treatment group, compared to 77.9 ± 9.1% in the bacterial treatment group after 24 h incubation. Findings of this study indicate that lytic phages might be utilized as potential bio-control agents of luminescent bacterial disease in oyster hatcheries.

Item Description

10.3390/antibiotics9070415
2079-6382