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Expression profile of plasma microRNAs and target genes in patients with complicated pregnancy

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Aim: to assess features of placental transcriptome in patients with complicated pregnancy course and outcome.Materials and Methods. A prospective observational comparative study in parallel groups was carried out by examining 44 patients divided into three groups: group 1 included 13 pregnant women...

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Main Authors: M. D. Umerova (Author), S. S. Alyadinova (Author), M. J. Khonjonova (Author), R. A. Balakadasheva (Author), E. E. Menadzhiev (Author), D. I. Kharchuk (Author), D. O. Leus (Author), G. I. Islyamova (Author), M. I. Islyamova (Author), S. L. Abkarimova (Author), D. S. Kasabyan (Author), L. E. Sorokina (Author)
Format: Book
Published: IRBIS LLC, 2023-07-01T00:00:00Z.
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Summary:Aim: to assess features of placental transcriptome in patients with complicated pregnancy course and outcome.Materials and Methods. A prospective observational comparative study in parallel groups was carried out by examining 44 patients divided into three groups: group 1 included 13 pregnant women with moderate and severe preeclampsia (PE), group 2 consisted of 12 patients with fetal growth retardation (FGR), control group included 19 clinically healthy women with uncomplicated pregnancy and delivery. All women enrolled to the study underwent a comprehensive examination, including history collection, general and obstetric-gynecological examination, laboratory and instrumental studies. Special research methods were used during the study, which included placental tissue sampling followed by analyzed expression profile of 12 microRNAs using real-time polymerase chain reaction (PCR).Results. The PCR data indicated about lack of expression for analyzed miR-210-3p, miR-320-3p, miR-1304-5p and miR-375-5p in placental tissue samples in FGR patients. No significant differences in placental miRNA levels were observed in FGR vs. PE and control group. Analysis of placenta-specific microRNAs in women with PE vs. control group showed a significantly down modulated expression level for miR-517a-3p (p = 0.025), miR-517c-3p (p = 0.036), miR-574-5p (p = 0.015) along with upregulated expression of miR-20a-5p (p = 0.046).Conclusion. The data obtained evidence that pregnancy-related complications are characterized by specific molecular changes at placental transcriptome level.
Item Description:2313-7347
2500-3194
10.17749/2313-7347/ob.gyn.rep.2023.420

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