Comparative Chemical Analysis of Eight <i>Punica granatum</i> L. Peel Cultivars and Their Antioxidant and Anti-Inflammatory Activities

• Главные авторы: Valentina Parisi (Автор), Valentina Santoro (Автор), Giuliana Donadio (Автор), Maria Laura Bellone (Автор), Gianfranco Diretto (Автор), Carla Sandri (Автор), Francesca Mensitieri (Автор), Nunziatina De Tommasi (Автор), Fabrizio Dal Piaz (Автор), Alessandra Braca (Автор)
• Формат: ‌
• Опубликовано: MDPI AG, 2022-11-01T00:00:00Z.
• Предметы: article
• Online-ссылка: Connect to this object online.
• Notes: 10.3390/antiox11112262; 2076-3921
• Keywords: <i>Punica granatum</i>; pomegranate; mass spectrometry; phenolic compounds; antioxidant; anti-inflammatory; Therapeutics. Pharmacology; RM1-950
• Source: Antioxidants, Vol 11, Iss 11, p 2262 (2022)

A comparative quali-quantitative study of the peel extracts of eight <i>Punica granatum</i> cultivars obtained from underexploited areas of South Italy was carried out in order to valorize them as health-promoting by-products. The results showed that all of the samples possessed 45 ellagitannins, consisting mainly of polyhydroxyphenoyls; 10 flavonoids, belonging to flavonol, flavone, and catechin classes; and 2 anthocyanins. The most representative compounds underwent quantification through a LC-MS/MS multiple reaction monitoring (MRM)-based method; their qualitative profile was almost superimposable, while variability in the quantitative phenolic content was observed. The antioxidant activity was investigated using cell-free and cell-based assays. The in vitro anti-inflammatory potential was also studied by monitoring three typical markers of inflammation (IL-1β, IL-6, and TNF-α). Moderate differences in both activities were observed between the cultivars. Results showed that all of the investigated peels have a potential use as healthy bioactive phytocomplexes due to the interesting antioxidant and anti-inflammatory activities; in particular from the bioinformatic approaches a series of compounds, including galloyl-, pedunculagin- and ellagic acid-based, were found to be highly correlated with bioactivity of the extracts. Finally, the bioactivities showed by a Campanian local cultivar, 'Granato di Aiello del Sabato', could promote its cultivation by local farmers and germplasm conservation.