Expression and clinical significance of miR-770-5p and miR-145-5p in patients with diabetic nephropathy

• Main Authors: Bing-fei Zhang (Author), Xue Sun (Author)
• Format: Book
• Published: Editorial Department of Journal of Clinical Nephrology, 2024-11-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Summary

Objective To explore the expressions and clinical significance of MicroRNA-770-5p （miR-770-5p） and MicroRNA-145-5p （miR-145-5p） in patients with diabetic kidney disease （DKD）. MethodsFrom December 2020 to December 2022, 132 DKD patients （n = 132） were designated as DKD group, another 132 type 2 diabetes patients （n = 132） as T2DM group and still another 132 health checkups at the same time as control group. Real-time fluorescent quantitative polymerase chain reaction （qRT-PCR） was applied for measuring the serum expression levels of miR-770-5p and miR-145-5p. The prognostic factors of DKD were examined by multiple Logistic regression analysis. And predictive value of serum miR-770-5p and miR-145-5p for DKD prognosis was analyzed through plotting receiver operating characteristic （ROC） curve. Results As compared with control group, the expression levels of serum miR-770-5p and miR-145-5p spiked markedly in T2DM and DKD groups （P＜0.05）. As compared with early DKD group, the serum expression levels of miR-770-5p and miR-145-5p in middle and late DKD groups rose obviously （P＜0.05）. As compared with good prognosis group, the serum expression levels of miR-770-5p and miR-145-5p jumped greatly in poor prognosis group （P＜0.05）. As compared with good prognosis group, there were significant differences in urea nitrogen, albumin and serum creatinine in poor prognosis group （P＜0.05）. Multivariate Logistic results indicated that miR-770-5p, miR-145-5p, urea nitrogen and serum creatinine were risk factors for the prognosis （P＜0.05） while albumin was a protective factor （P＜0.05）. The area under the curve （AUC） of the combined prognostic prediction of serum miR-770-5p and miR-145-5p was the highest. It was better than individual prediction of serum miR-770-5p and miR-145-5p （Z combination - miR-770-5p = 3.632, P = 0.003, Z combination - miR-145-5p = 2.370, P = 0.018）. Conclusions The serum expression levels of miR-770-5p and miR-145-5p are up-regulated in DKD patients. It is closely correlated with the severity and prognosis of DKD. The combination of the two is more valuable in evaluating the prognosis of DKD patients.

Item Description

1671-2390
10.3969/j.issn.1671-2390.2024.11.002