Respon inflamasi pulpa gigi tikus Sprague Dawley setelah aplikasi bahan etsa ethylene diamine tetraacetic acid 19% dan asam fosfat 37%

• Main Authors: Nadie Fatimatuzzahro (Author), Tetiana Haniastuti (Author), Juni Handajani (Author)
• Format: Book
• Published: Universitas Airlangga, 2013-12-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Summary

<p><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Background: </strong><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>Etching agents such as ethylene diamine tetraacetic acid (EDTA) and phosphoric acid which are widely used in </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>adhesive restoration system, are aimed to increase retention of restorative materials; however, these agents may induce inflammation </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>of dental pulp. The major function of the inflammatory response is to remove invading pathogens or damaged tissue/ cells and </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>therefore, initiate repair. Neutrophils and macrophages are motile phagocytes that constitute the body's first line of defense. </em><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Purpose: </strong><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>The purpose of the present research was to study the effect of 19% EDTA and 37% phosphoric acid for etching application agents </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>on the inflammatory response of the dental pulp. </em><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Methods: </strong><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>Forty-five male Sprague Dawley rats were divided into 3 groups. Cavity </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>preparation was made on the occlusal surface of maxillary first molar using a round diamond bur. Nineteen percent of EDTA, 37% </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>phosphoric acid, and distilled water were applied on the surface of the cavity of the teeth in group I, II and III respectively. The rats </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>were sacrified at 1, 3, 5, 7, and 14 days after the application (n=3 for each day). The specimens were then processed histologically </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>and stained with hematoxylin eosin. </em><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Results: </strong><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>ANOVA showed a significant difference (p<0.05) among treatment groups, indicating </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>that etching agents application induced neutrophils, macrophages and lymphocytes infiltration in the dental pulp. Tuckey HSD test </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>showed that application of 37% phosphoric acid increased higher number of neutrophils, macrophages and lymphocytes significantly </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>than 19% EDTA (p<0.05). </em><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Conclusion: </strong><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>The study suggested that 37% phosphoric acid induced higher number of the inflammatory </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>cells than 19% EDTA.</em></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></p><p><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Latar belakang: </strong><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>Penggunaan bahan etsa seperti ethylene diamine tetraacetic acid (EDTA) dan asam fosfat pada sistem restorasi </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>adhesif bertujuan untuk meningkatkan retensi bagi bahan restorasi, namun penggunaan bahan-bahan tersebut dapat menginduksi </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>inflamasi pada pulpa. Respon inflamasi berfungsi untuk menghilangkan patogen, sel-sel atau jaringan yang rusak dan menginisiasi </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>perbaikan. Netrofil dan makrofag adalah sel fagosit yang merupakan garis pertama pertahanan tubuh. </em><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Tujuan: </strong><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>Penelitian ini bertujuan </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>untuk meneliti efek EDTA 19% dan asam fosfat 37% sebagai bahan etsa terhadap respon inflamasi pada pulpa gigi. </em><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Metode: </strong><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>Empat </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>puluh lima ekor tikus Sprague Dawley jantan dibagi menjadi 3 kelompok. Permukaan oklusal gigi molar satu rahang atas dipreparasi </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>menggunakan diamond round bur. Pada kelompok I kavitas diaplikasikan EDTA 19%, kelompok II diaplikasikan asam fosfat 37% </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>dan kelompok III diaplikasikan akuades. Hewan coba dikorbankan pada hari ke-1, 3, 5, 7 dan 14 setelah aplikasi bahan etsa (n=3).
</em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>Spesimen diproses secara histologis dan dicat dengan hematoksilin eosin. </em><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Hasil: </strong><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>Hasil ANOVA menunjukkan perbedaan yang bermakna</em></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span> <span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>(p<0,05) antar kelompok perlakuan, mengindikasikan bahwa aplikasi bahan etsa menyebabkan infiltrasi sel inflamasi pada pulpa, </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>baik netrofil, makrofag dan limfosit. Hasil uji Tuckey HSD menunjukkan bahwa asam fosfat 37% menstimulasi infiltrasi sel netrofil, </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>makrofag dan limfosit signifikan (p<0,05) lebih banyak dibanding EDTA 19%. </em><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Simpulan: </strong><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>Penelitian ini menunjukkan bahwa asam </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>fosfat 37% menyebabkan infiltrasi sel inflamasi yang lebih banyak dibanding EDTA 19%.</em></span></span></span></span></span></span><br style="font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-align: -webkit-auto; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px;" /></span></span></span></span></span></span></span></span></span></p>

Item Description

1978-3728
2442-9740
10.20473/j.djmkg.v46.i4.p190-195