TNF-alpha expression, evaluation of collagen, and TUNEL of Matricaria recutita L. extract and triamcinolone on oral ulcer in diabetic rats

• Main Authors: Bruna Vasconcelos OLIVEIRA (Author), Paulo Goberlânio BARROS SILVA (Author), Jacqueline de Santiago NOJOSA (Author), Luiz André Cavalcante BRIZENO (Author), Jamile Magalhães FERREIRA (Author), Fabrício Bitú SOUSA (Author), Mário Rogério Lima MOTA (Author), Ana Paula Negreiros Nunes ALVES (Author)
• Format: Book
• Published: University of São Paulo.
• Subjects: article
• Online Access: Connect to this object online.
• Notes: 1678-7765; 10.1590/1678-775720150481
• Keywords: Oral ulcer; Diabetes mellitus; Chamomile; Matricaria; Triamcinolone; Dentistry; RK1-715
• Source: Journal of Applied Oral Science, Vol 24, Iss 3, Pp 278-290

ABSTRACT Diabetes mellitus (DM) is a disease associated with delayed wound healing of oral ulcers by increased expression of proinflammatory cytokines and cellular apoptosis. Objective to evaluate the influence of Tumor Necrosis Factor alpha (TNF-α) and apoptosis in rats with DM treated with chamomile extract or triamcinolone. Material and Methods Wistar male rats (210.0±4.2 g) were divided into five groups: negative control group (NCG) without diabetes; positive control group (PCG) with DM (alloxan, 45 mg/kg); and groups treated with chamomile extract (normoglycemic= NCG group and diabetic= DCG group) and with triamcinolone (TG). Traumatic ulcers were performed on all animals that received topical triamcinolone, chamomile extract or saline 12/12 hours for ten days. Results On days five and ten the animals were euthanized and the ulcers were analyzed by light microscopy, TUNEL assay, and immunohistochemically (TNF-α). The NCG (p=0.0062), PCG (p=0.0285), NCG (p=0.0041), and DCG (p<0.0001) groups were completely healed on the 10th day, however, there was no healing on the TG (p=0.5127) group. The TNF-α expression showed a significant reduction from the 5th to the 10th day in NCG (p=0.0266) and DCG (p=0.0062). In connective tissue, the TUNEL assay showed a significant reduction in the number of positive cells in NCG (p=0.0273) and CNG (p=0.0469) and in the epithelium only in CDG (p=0.0320). Conclusions Chamomile extract can optimize the healing of traumatic oral ulcers in diabetic rats through the reduction of apoptosis in the epithelium and TNF-α expression.