HIV-1 Low-Frequency Variants Identified in Antiretroviral-Naïve Subjects with Virologic Failure after 12 Months of Follow-Up in Panama
Low-frequency mutations associated with drug resistance have been related to virologic failure in subjects with no history of pre-treatment and recent HIV diagnosis. In total, 78 antiretroviral treatment (ART)-naïve subjects with a recent HIV diagnosis were selected and followed by CD4+ T lymphocyte...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Book |
| Published: |
MDPI AG,
2023-08-01T00:00:00Z.
|
| Subjects: | |
| Online Access: | Connect to this object online. |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
MARC
| LEADER | 00000 am a22000003u 4500 | ||
|---|---|---|---|
| 001 | doaj_fea2d72742514cb0a9dba384ecbc2f85 | ||
| 042 | |a dc | ||
| 100 | 1 | 0 | |a Ambar Moreno |e author |
| 700 | 1 | 0 | |a Claudia González |e author |
| 700 | 1 | 0 | |a Jessica Góndola |e author |
| 700 | 1 | 0 | |a Oris Chavarría |e author |
| 700 | 1 | 0 | |a Alma Ortiz |e author |
| 700 | 1 | 0 | |a Jorge Castillo |e author |
| 700 | 1 | 0 | |a Juan Castillo Mewa |e author |
| 700 | 1 | 0 | |a Juan Miguel Pascale |e author |
| 700 | 1 | 0 | |a Alexander Augusto Martínez |e author |
| 245 | 0 | 0 | |a HIV-1 Low-Frequency Variants Identified in Antiretroviral-Naïve Subjects with Virologic Failure after 12 Months of Follow-Up in Panama |
| 260 | |b MDPI AG, |c 2023-08-01T00:00:00Z. | ||
| 500 | |a 10.3390/idr15040044 | ||
| 500 | |a 2036-7449 | ||
| 520 | |a Low-frequency mutations associated with drug resistance have been related to virologic failure in subjects with no history of pre-treatment and recent HIV diagnosis. In total, 78 antiretroviral treatment (ART)-naïve subjects with a recent HIV diagnosis were selected and followed by CD4+ T lymphocytes and viral load tests to detect virologic failure. We sequenced the basal samples retrospectively using next-generation sequencing (NGS), looking for low-frequency mutations that had not been detected before using the Sanger sequencing method (SSM) and describing the response to ART. Twenty-two subjects developed virologic failure (VF), and thirteen of them had at least one drug-resistance mutation associated with Reverse Transcriptase Inhibitors (RTI) and Protease Inhibitors (PIs) at frequency levels ≤ 1%, not detected previously in their basal genotyping test. No resistance mutations were observed to Integrase Strand Transfer Inhibitors (INSTIs). We identified a possible cause of VF in ART-naïve subjects with low-frequency mutations detected. To our knowledge, this is the first evaluation of pre-existing drug resistance for HIV-1 minority variants carried out on ART-naïve people living with HIV/AIDS (PLWHA) by analyzing the HIV-1 <i>pol</i> gene using NGS in the country. | ||
| 546 | |a EN | ||
| 690 | |a HIV-1 | ||
| 690 | |a low-frequency variants | ||
| 690 | |a virologic failure | ||
| 690 | |a next-generation sequencing | ||
| 690 | |a drug resistance | ||
| 690 | |a Panama | ||
| 690 | |a Other systems of medicine | ||
| 690 | |a RZ201-999 | ||
| 655 | 7 | |a article |2 local | |
| 786 | 0 | |n Infectious Disease Reports, Vol 15, Iss 4, Pp 436-444 (2023) | |
| 787 | 0 | |n https://www.mdpi.com/2036-7449/15/4/44 | |
| 787 | 0 | |n https://doaj.org/toc/2036-7449 | |
| 856 | 4 | 1 | |u https://doaj.org/article/fea2d72742514cb0a9dba384ecbc2f85 |z Connect to this object online. |