Global profiling of protein lysine malonylation in Toxoplasma gondii strains of different virulence and genetic backgrounds.

Lysine malonylation is a post-translational modification (PTM), which regulates many cellular processes. Limited information is available about the level of lysine malonylation variations between Toxoplasma gondii strains of distinct genetic lineages. Yet, insights into such variations are needed to...

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Main Authors: Lan-Bi Nie (Author), Qin-Li Liang (Author), Meng Wang (Author), Rui Du (Author), Meng-Yuan Zhang (Author), Hany M Elsheikha (Author), Xing-Quan Zhu (Author)
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Published: Public Library of Science (PLoS), 2022-05-01T00:00:00Z.
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100 1 0 |a Lan-Bi Nie  |e author 
700 1 0 |a Qin-Li Liang  |e author 
700 1 0 |a Meng Wang  |e author 
700 1 0 |a Rui Du  |e author 
700 1 0 |a Meng-Yuan Zhang  |e author 
700 1 0 |a Hany M Elsheikha  |e author 
700 1 0 |a Xing-Quan Zhu  |e author 
245 0 0 |a Global profiling of protein lysine malonylation in Toxoplasma gondii strains of different virulence and genetic backgrounds. 
260 |b Public Library of Science (PLoS),   |c 2022-05-01T00:00:00Z. 
500 |a 1935-2727 
500 |a 1935-2735 
500 |a 10.1371/journal.pntd.0010431 
520 |a Lysine malonylation is a post-translational modification (PTM), which regulates many cellular processes. Limited information is available about the level of lysine malonylation variations between Toxoplasma gondii strains of distinct genetic lineages. Yet, insights into such variations are needed to understand the extent to which lysine malonylation contributes to the differences in the virulence and repertoire of virulence factors between T. gondii genotypes. In this study, we profiled lysine malonylation in T. gondii using quantitative liquid chromatography-tandem mass spectrometry (LC-MS/MS) and immuno-affinity purification. This analysis was performed on three T. gondii strains with distinctive pathogenicity in mice, including RH strain (type I), PRU strain (type II), and VEG strain (type III). In total, 111 differentially malonylated proteins and 152 sites were upregulated, and 17 proteins and 17 sites were downregulated in RH strain versus PRU strain; 50 proteins and 59 sites were upregulated, 50 proteins and 53 sites were downregulated in RH strain versus VEG strain; and 72 proteins and 90 sites were upregulated, and 7 proteins and 8 sites were downregulated in VEG strain versus PRU strain. Differentially malonylated proteins were involved in key processes, such as those mediating the regulation of protein metabolism, stress response, glycolysis, and actin cytoskeleton. These results reveal an association between lysine malonylation and intra-species virulence differences in T. gondii and offer a new resource for elucidating the contribution of lysine malonylation to energy metabolism and virulence in T. gondii. 
546 |a EN 
690 |a Arctic medicine. Tropical medicine 
690 |a RC955-962 
690 |a Public aspects of medicine 
690 |a RA1-1270 
655 7 |a article  |2 local 
786 0 |n PLoS Neglected Tropical Diseases, Vol 16, Iss 5, p e0010431 (2022) 
787 0 |n https://doi.org/10.1371/journal.pntd.0010431 
787 0 |n https://doaj.org/toc/1935-2727 
787 0 |n https://doaj.org/toc/1935-2735 
856 4 1 |u https://doaj.org/article/ff20194f3dba40e8bb095f6375dacdf1  |z Connect to this object online.