Genetic deletion of Cav3.2 T-type calcium channels abolishes H2S-dependent somatic and visceral pain signaling in C57BL/6 mice
We tested whether genetic deletion of Cav3.2 T-type Ca2+ channels abolishes hydrogen sulfide (H2S)-mediated pain signals in mice. In Cav3.2-expressing HEK293 cells, Na2S, an H2S donor, at 100 μM clearly increased Ba2+ currents, as assessed by whole-cell patch-clamp recordings. In wild-type C57BL/6 m...
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Elsevier,
2019-07-01T00:00:00Z.
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| 042 | |a dc | ||
| 100 | 1 | 0 | |a Kazuki Matsui |e author |
| 700 | 1 | 0 | |a Maho Tsubota |e author |
| 700 | 1 | 0 | |a Saaya Fukushi |e author |
| 700 | 1 | 0 | |a Nene Koike |e author |
| 700 | 1 | 0 | |a Hiroshi Masuda |e author |
| 700 | 1 | 0 | |a Yoshihito Kasanami |e author |
| 700 | 1 | 0 | |a Takaya Miyazaki |e author |
| 700 | 1 | 0 | |a Fumiko Sekiguchi |e author |
| 700 | 1 | 0 | |a Tsuyako Ohkubo |e author |
| 700 | 1 | 0 | |a Shigeru Yoshida |e author |
| 700 | 1 | 0 | |a Yutaro Mukai |e author |
| 700 | 1 | 0 | |a Akira Oita |e author |
| 700 | 1 | 0 | |a Mitsutaka Takada |e author |
| 700 | 1 | 0 | |a Atsufumi Kawabata |e author |
| 245 | 0 | 0 | |a Genetic deletion of Cav3.2 T-type calcium channels abolishes H2S-dependent somatic and visceral pain signaling in C57BL/6 mice |
| 260 | |b Elsevier, |c 2019-07-01T00:00:00Z. | ||
| 500 | |a 1347-8613 | ||
| 500 | |a 10.1016/j.jphs.2019.07.010 | ||
| 520 | |a We tested whether genetic deletion of Cav3.2 T-type Ca2+ channels abolishes hydrogen sulfide (H2S)-mediated pain signals in mice. In Cav3.2-expressing HEK293 cells, Na2S, an H2S donor, at 100 μM clearly increased Ba2+ currents, as assessed by whole-cell patch-clamp recordings. In wild-type C57BL/6 mice, intraplantar and intracolonic administration of Na2S evoked mechanical allodynia and visceral nociceptive behavior, respectively, which were abolished by TTA-A2, a T-type Ca2+ channel blocker. In Cav3.2-knockout mice of a C57BL/6 background, Na2S caused neither somatic allodynia nor colonic nociception. Our study thus provides definitive evidence for an essential role of Cav3.2 in H2S-dependent somatic and colonic pain. Keywords: Hydrogen sulfide, Cav3.2 T-type calcium channel, Pain | ||
| 546 | |a EN | ||
| 690 | |a Therapeutics. Pharmacology | ||
| 690 | |a RM1-950 | ||
| 655 | 7 | |a article |2 local | |
| 786 | 0 | |n Journal of Pharmacological Sciences, Vol 140, Iss 3, Pp 310-312 (2019) | |
| 787 | 0 | |n http://www.sciencedirect.com/science/article/pii/S1347861319356907 | |
| 787 | 0 | |n https://doaj.org/toc/1347-8613 | |
| 856 | 4 | 1 | |u https://doaj.org/article/ff3f7db3b8f64ae19327cfa7749e3fc8 |z Connect to this object online. |