Efficacy of Copper Ion Treatment on Bacteria and Antibiotic Residues Contained in Bovine Waste Milk

• Principais autores: Fernando Ulloa (Autor), Martina Penati (Autor), Constanza Naegel (Autor), Carlos Tejeda (Autor), Miguel Hernández-Agudelo (Autor), Pamela Steuer (Autor), Miguel Salgado (Autor)
• Formato: Livro
• Publicado em: MDPI AG, 2024-11-01T00:00:00Z.
• Assuntos: article
• Acesso em linha: Connect to this object online.
• Notes: 10.3390/antibiotics13111085; 2079-6382
• Keywords: waste milk; copper ion treatment; microorganisms; antibiotics; Therapeutics. Pharmacology; RM1-950
• Source: Antibiotics, Vol 13, Iss 11, p 1085 (2024)

<b>Background/Objectives</b>: Waste milk harbors many bacteria and antibiotic residues. Calves fed with untreated waste milk have a higher incidence of scours and an increased risk of developing antimicrobial-resistant bacteria. This study aimed to evaluate the efficacy of treatment with copper ions on bacteria and antibiotics contained in bovine waste milk. <b>Methods</b>: Waste milk samples were collected from a dairy farm for seven weeks and were subjected to treatment with copper ions. Total bacterial counts, coliforms, <i>Streptococcus</i>, and <i>Staphylococcus</i> were assessed before and after treatment. Additionally, metagenomic analysis was performed to determine microbial diversity. <b>Results</b>: Before treatment, the total bacterial count average was 4.0 × 10⁶ CFU/mL, 1.7 × 10⁴ CFU/mL for coliforms, 2.6 × 10⁶ CFU/mL for <i>Streptococcus</i>, and 5.4 × 10² CFU/mL for <i>Staphylococcus</i> Copper treatment significantly reduced bacterial counts within 15 min. Total bacteria decreased from 4.0 × 10⁶ CFU/mL to 1.1 × 10² CFU/mL after 30 min; meanwhile, other groups were not detected. The most abundant groups were <i>Lactococcus</i> (29.94%), <i>Pseudomonas</i> (28.89%), and <i>Enterobacteriaceae</i> (21.19%). Beta-lactams were detected in five-sevenths samples, and in one sample they were detected before and at 15 min of treatment but not after 30 min. <b>Conclusions</b>: The effect of treatment with copper ions on the different bacterial groups was significantly effective but showed limited effect on the detection of antibiotics.