Genomic Analysis of CTX-M-Group-1-Producing Extraintestinal Pathogenic <i>E. coli</i> (ExPEc) from Patients with Urinary Tract Infections (UTI) from Colombia
Background: The dissemination of the uropathogenic O25b-ST131 <i>Escherichia coli</i> clone constitutes a threat to public health. We aimed to determine the circulation of <i>E. coli</i> strains belonging to O25b:H4-B2-ST131 and the <i>H</i>30-Rx epidemic subclone...
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| Main Authors: | , , , , , , , |
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| Format: | Book |
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MDPI AG,
2020-12-01T00:00:00Z.
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| Summary: | Background: The dissemination of the uropathogenic O25b-ST131 <i>Escherichia coli</i> clone constitutes a threat to public health. We aimed to determine the circulation of <i>E. coli</i> strains belonging to O25b:H4-B2-ST131 and the <i>H</i>30-Rx epidemic subclone causing hospital and community-acquired urinary tract infections (UTI) in Colombia. Methods: Twenty-six nonduplicate, CTX-M group-1-producing isolates causing UTI in the hospital and community were selected for this study. Results: Twenty-two <i>E. coli</i> isolates harboring CTX-M-15, one CTX-M-3, and three CTX-M-55 were identified. Multilocus Sequence Typing (MLST) showed a variety of sequence types (STs), among which, ST131, ST405, and ST648 were reported as epidemic clones. All the <i>E. coli</i> ST131 sequences carried CTX-M-15, from which 80% belonged to the O25b:H4-B2 and <i>H</i>30-Rx pandemic subclones and were associated with virulence factors <i>iss</i>, <i>iha</i>, and <i>sat</i>. <i>E. coli</i> isolates (23/26) were resistant to ciprofloxacin and associated with amino acid substitutions in quinolone resistance-determining regions (QRDR). We detected two carbapenem-resistant <i>E. coli</i> isolates, one coproducing CTX-M-15, KPC-2, and NDM-1 while the other presented mutations in <i>ompC</i>. Additionally, one isolate harbored the gene <i>mcr-1</i>. Conclusions: Our study revealed the circulation of the <i>E. coli</i> ST131, O25b:H4-B2-<i>H</i>30-Rx subclone, harboring CTX-M-15, QRDR mutations, and other resistant genes. The association of the <i>H</i>30-Rx subclone with sepsis and rapid dissemination warrants attention from the public health and infections control. |
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| Item Description: | 10.3390/antibiotics9120899 2079-6382 |