Study on the cytotoxicity and chromosome aberration following implantation of sea coral in rabbits
<span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>Coral has been used as a bone substitute in many experimental studies. It has been proven to be biocompatible, biodegradable </em><span style="f...
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| Format: | Book |
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Universitas Airlangga,
2006-03-01T00:00:00Z.
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| Summary: | <span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>Coral has been used as a bone substitute in many experimental studies. It has been proven to be biocompatible, biodegradable </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>and easy to handle: and it has not been found to cause any inflammatory responses. The present study was undertaken to determine </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>the cytotoxicity in terms of mitotic index as well as the clastogenic effect (chromosome aberration) of sea coral implantation in </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>rabbits. The animals comprised of five male adult healthy New Zealand White (Oryctolagus cuniculus) rabbits. The biomaterial, sea </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>coral granules used in this study was obtained from Porites species and processed by the tissue bank of Universiti Sains Malaysia, </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>Health Campus, Kubang Kerian, Malaysia. The blood samples were collected twice from the rabbits, once before the implantation of </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>the sea coral granules (which acted as the control) and the other, one week after the implantation (which acted as the treatment) and </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>lymphocyte cultures were set up. The cultures were then harvested and the chromosomes were prepared for analysis. The diploid </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>number of chromosomes in the rabbits (Oryctolagus cuniculus) was found to be 44. Mean mitotic indices of 3.84 ± 0.54 per cent and </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>3.76 ± 0.23 per cent were obtained before and after implantation of sea coral granules respectively. There were no structural or </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>numerical chromosomal aberrations observed in both the cases. The mitotic index values and chromosomal analyses in this preliminary </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>study carried out indicate that the biomaterial, sea coral granules is non-cytotoxic and non-clastogenic under the present test </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>conditions.</em></span></span></span></span></span></span></span></span></span></span></span></span></span> |
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| Item Description: | 1978-3728 2442-9740 10.20473/j.djmkg.v39.i1.p8-11 |