Local Administration of Insulin-like Growth Factor I into Silicon Rubber Chamber Improves Peripheral Nerve Repair in Rats
<p><strong>Introduction:</strong> Organ reinnervation and functional recovery following peripheral nerve injury still remains a major challenge and return of functional recovery to the preinjured level rarely occurs.The aim was to evaluate the effect of locally administered insulin...
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| Main Authors: | , , , |
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| Format: | Book |
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Journal of Surgery and Surgical Research - Peertechz Publications,
2015-04-13.
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| Online Access: | Connect to this object online. |
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| Summary: | <p><strong>Introduction:</strong> Organ reinnervation and functional recovery following peripheral nerve injury still remains a major challenge and return of functional recovery to the preinjured level rarely occurs.The aim was to evaluate the effect of locally administered insulin-like growth factor (IGF I) on nerve regeneration.<strong><br></strong></p><p><strong>Methods:</strong> Eighty male white Wistar rats were divided into four groups (n = 20), randomly: Intransected group (TC), left sciatic nerve was transected and stumps were fixed in the adjacent muscle.In treatment group defect was bridged using a silicone conduit (SIL/IGF) and filled with 10 μL IGF I (100 ng/kg). In silicone graft group (SIL), the graft was filled with phosphate-buffered saline alone. In shamoperated group (SHAM), sciatic nerve was exposed and manipulated. Each group was subdivided into three subgroups of five animals each and studied 4, 8 and 12 weeks after surgery.</p><p><strong>Results:</strong> Behavioral testing, sciatic nerve functional study, gastrocnemius muscle mass and morphometric indices confirmed faster recovery of regenerated axons in SIL/IGF than in SIL group (p< 0.05). In immunohistochemistry, location of reactions to S-100 in SIL/IGF was clearly more positive than that in SIL group.</p><p><strong>Conclusion:</strong> When loaded in a silicone graft, IGF I accelerated and improved functional recovery and morphometric indices of sciatic nerve. Thus, dose-response studies should be conducted for IGF I to determine the combination of the graft and the compound that achieve maximal efficacy in nerve transection models.</p> |
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| DOI: | 10.17352/2455-2968.000002 |