C-Kit Positive Cells from Failing Human Hearts: Role of Culturing Media on Cardiomyogenic Potentials

• Main Authors: Anna Francesconi (Author), Gaetano Paparella (Author), Barbara Frossi (Author), Elisa Del Terra (Author), Francesco Saverio Ambesi Impiombato (Author), Francesco Curcio (Author)
• Format: Book
• Published: Journal of Cardiovascular Medicine and Cardiology - Peertechz Publications, 2015-03-07.
• Subjects: Research Article
• Online Access: Connect to this object online.

<p><strong>Background: </strong>The possibility of culturing heart cells in order to regenerate damaged tissue is a challenging problem. Recent observations have demonstrated the possibility of isolating and expanding resident cardiac stem cells, which could favor regeneration and functional improvement of the myocardial tissue.</p><p><strong>Aims:</strong> To investigate two different culturing media: one promoting c-kit cells' growth and the other promoting differentiation in cardiac muscle cells.</p><p><strong>Methods: </strong>We obtained primary cultures from left ventricle myocardial tissues of 10 human hearts of patients with end-stage heart failure who received heart transplantation. Cells were first cultured in a medium containing high serum and low calcium/magnesium (Ca2+ /Mg2+) to promote cell growth (medium A). Than they were cultured in another medium that contained lower serum concentration and a variety of different factors in order to induce cell differentiation (medium B). The presence of c-kit, specific for stem cells, α-sarcomeric actin (SA), specific for skeletal and cardiac muscle cells, and α-smooth muscle actin (SMA), specific for smooth muscle cells was studied by immune-cytochemical analysis.</p><p><strong>Results: </strong>A high percentage of c-kit+, SMA-, SA- cells was observed in medium A; in medium B with lower serum and higher Ca2+/Mg2+ concentrations cells became c-kit-, AML+, SA+. In medium A, 78% of the cells were positive for c-kit. After culturing the same cell populations in medium B with lower serum and higher Ca2+ /Mg2+ concentrations, the percentage of c-kit positive cells decreased to 21% while the cells positive for SMA and for SA increased respectively from 28 to 82% and from 0 to 59%.</p><p><strong>Conclusions: </strong>Our results confirm the presence of a high percentage of c-kit positive cells in failing human myocardium and, for the first time, suggest a key role of calcium/magnesium concentration in promoting both c-kit cells' growth and their differentiation in human cardiac muscle.</p>