The effects of thymoquinone and cytozine arabinoside on apoptosis and cell proliferation in acute myeloide leukemia

• Main Authors: Aslı Altun (Author), Nurten Kara (Author), Şengül Tural (Author), Alişan Yıldıran (Author), Leman Tomak (Author)
• Format: Book
• Published: Global Journal of Cancer Therapy - Peertechz Publications, 2022-10-11.
• Subjects: Research Article
• Online Access: Connect to this object online.

<p>Purpose: The aim of this study was to investigate the effects of a chemotherapeutic agent Cytosine Arabinoside (Ara-C) and a natural anticancer agent of Thymoquinone (TQ) on apoptosis and cell proliferation of AML cell lines (Kasumi-6) both alone and in combined form.</p><p>Material and method: Kasumi-6 AML cells were treated with three different doses of Ara-C (0.1, 0.5 and 1 µmol) and TQ (25, 50 and 100 µM) for 48 and 72 hours incubations. After Annexin V and Propidium Iodide (PI) staining, apoptosis, viability, and cell proliferation were evaluated for each group in flow cytometry.</p><p>Results: As a result, AML cell lines showed a statistically significant difference in a single treatment of the active substances. Their combined treatment showed an increase in apoptosis and a decrease in viability in both groups at 48 and 72 hours incubation times (p < 0.001). In each group, it was observed that apoptosis was increased and viability was decreased and consequently cell proliferation was suppressed.</p><p>Conclusion: Ara-C was used for the first time in this study with TQ in AML. It was determined that the combined use of TQ and Ara-C did not have a synergistic effect on apoptosis.</p>