Important Factors Influencing Protein Crystallization
<p>The solution of crystallization problem was introduced around twenty years ago, with the introduction of crystallization screening methods. Here reported some of the factors which affect protein crystallization, solubility, Concentration of precipitant, concentration of macromolecule, ionic...
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| Main Authors: | , , , |
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| Format: | Book |
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Global Journal of Biotechnology and Biomaterial Science - Peertechz Publications,
2016-09-30.
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| Online Access: | Connect to this object online. |
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| Summary: | <p>The solution of crystallization problem was introduced around twenty years ago, with the introduction of crystallization screening methods. Here reported some of the factors which affect protein crystallization, solubility, Concentration of precipitant, concentration of macromolecule, ionic strength, pH, temperature, and organism source of macromolecules, reducing or oxidizing environment,additives, ligands, presence of substrates, inhibitors, coenzymes, metal ions and rate of equilibration.The aim of this paper to give very helpful advice for crystallization.</p> |
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| DOI: | 10.17352/gjbbs.000008 |