Correlation Analysis between mtDNA G10398A Polymorphism and Susceptibility of Patients with Type 2 Diabetes Mellitus in Northwest China

• Main Authors: Ting Ji (Author), Chen Tian (Author), Xin-Zhong Ji (Author), Li Huang (Author), Yun-Cong Wang (Author), Han Liu (Author), Xiao-Dong Xie (Author)
• Format: Book
• Published: Open Journal of Biological Sciences - Peertechz Publications, 2016-05-04.
• Subjects: Research Article
• Online Access: Connect to this object online.

<p><strong>Introduction:</strong> The present work aimed to investigate the association between G10398A polymorphism in mtDNA with type 2 diabetes mellitus (T2DM) susceptibility among Chinese northwestern population.</p><p><strong>Methods:</strong> Polymerase chain reaction followed by the restriction digestion of the amplicon to clarify the restriction fragment length polymorphism (PCR-RFLP) was used to score the G10398A variation of mtDNA NADH dehydrogenase3 (ND3) locus in a case-control study (n=422), including 209 patients with T2DM and 213 healthy controls.</p><p><strong>Results:</strong> The genotypes of G10398A were AA, AG, GG and the frequencies of genotypes and allele distributions were not significantly different in T2DM patients compared to healthy controls (P>0.05, χ2 test). There were no significant differences between genotypes and clinic indicators (P>0.05, single factor analysis of variance).</p><p><strong>Conclusions:</strong> The findings indicated that G10398A SNPs in ND3 locus of mtDNA were not in association with T2DM susceptibility in this population.</p>