Pemanfaatan Sumber Karbohidrat Yang Berbeda (Umbi Suweg, Umbi Talas, Dan Umbi Kimpul) Sebagai Substitusi Media Na (Nutrient Agar) Untuk Pertumbuhan Bakteri

• Main Author: Purwati, Suci (Author)
• Format: Book
• Published: 2016.
• Subjects: Thesis; NonPeerReviewed
• Online Access: Connect to this object online
• Notes: https://eprints.ums.ac.id/42281/1/10.%20ARTIKEL%20PUBLIKASI.pdf; https://eprints.ums.ac.id/42281/4/1.%20HALAMAN%20DEPAN.pdf; https://eprints.ums.ac.id/42281/7/2.%20BAB%20I.pdf; https://eprints.ums.ac.id/42281/10/3.%20BAB%20II.pdf; https://eprints.ums.ac.id/42281/11/4.%20BAB%20III.pdf; https://eprints.ums.ac.id/42281/15/5.%20BAB%20IV.pdf; https://eprints.ums.ac.id/42281/16/6.%20BAB%20V.pdf; https://eprints.ums.ac.id/42281/17/7.%20DAFTAR%20PUSTAKA.pdf; https://eprints.ums.ac.id/42281/18/8.%20LAMPIRAN.pdf; https://eprints.ums.ac.id/42281/19/PERNYATAAN%20PUBLIKASI%20KARYA%20ILMIAH.pdf
• Keywords: QR Microbiology

Instant media are difficult to obtain and relatively high prices encourage researchers to make a substitution media with materials that are cheap and easily. This studyaims to determinethe useof differentcarbohydrate sourcesassubstitutesmediaNutrientAgar (NA) forbacterial growth. Thisresearchusingcompletely randomized design(CRD) twofactors.The first factoris a typeof bacteriaisEscherichiacoliandStaphylococcusaureusandsecond is theAmorphophallus campanulatus, Colocasia esculenta, andXanthosoma violaceum. Inoculatedwith thetest bacteriaspreadplatemethodandincubatedat 37 °C for 24 hoursfor thebacteria inmediaNutrientagar and48 hourson mediumtubers.Parametersmeasured werethe number ofpopulationandthe size ofbacterial colonies.In Escherichiacoli, the highestpopulationon themediaof theAmorphophallus campanulatus (4.6 x 109CFU/ml), nutrient agar medium(3.6 x 109CFU/ml), Xanthosoma violaceum (3.5 x109CFU/ml), than the last Colocasia esculenta (2.8 x 109CFU/ml), while thehighestpopulation ofStaphylococcus aureuson theAmorphophallus campanulatus (8.2 x 109CFU/ml), nutrient agarmedia(5.5 x 109CFU/ml), Colocasia esculenta (3, 4x 109CFU/ml), and the last Xanthosoma violaceum (2.3 x 109CFU/ml). Based onthe size of thebacterial colonies, ofthe threemediatubersobtainsmallcolonybuta lot. The conclusion ofthis studymediathat can be usedas asubstitutenutrient agarmedium, ismediaofAmorphophallus campanulatus.