POTENSI ANTI QUORUM SENSING DARI EKSTRAK KASAR SUPERNATAN BAKTERI ENDOFIT Ageratum conyzoides

• Main Author: Desriana, Dora Syakina (Author)
• Format: Book
• Published: 2015-01-23.
• Subjects: Thesis; NonPeerReviewed
• Online Access: Link Metadata
• Notes: http://repository.upi.edu/16762/3/S_BIO_1000367_Title.pdf; http://repository.upi.edu/16762/4/S_BIO_1000367_Abstract.pdf; http://repository.upi.edu/16762/2/S_BIO_1000367_Table_of_content.pdf; http://repository.upi.edu/16762/5/S_BIO_1000367_Chapter1.pdf; http://repository.upi.edu/16762/5/S_BIO_1000367_Chapter2.pdf; http://repository.upi.edu/16762/6/S_BIO_1000367_Chapter3.pdf; http://repository.upi.edu/16762/1/S_BIO_1000367_Chapter4.pdf; http://repository.upi.edu/16762/1/S_BIO_1000367_Chapter5.pdf; http://repository.upi.edu/16762/4/S_BIO_1000367_Bibliography.pdf
• Keywords: L Education (General); QR Microbiology

Telah dilakukan penelitian mengenai ekstrak kasar supernatan bakteri endofit dari akar A. conyzoides sebagai anti quorum sensing. Quorum sensing adalah mekanisme komunikasi antarsel bakteri yang dapat mengatur fungsi fisiologi seperti biofilm, faktor virulensi dan persebaran bakteri. Tiga isolat bakteri endofit Ageratum conyzoides telah berhasil diisolasi yaitu Pseudomonas, Brochothrix, dan Kurthia. Penelitian ini bertujuan mengetahui potensi ekstrak kasar supernatan bakteri endofit A.conyzoides sebagai anti quorum sensing. Tiga isolat bakteri endofit dikultur dalam medium King's B hingga mencapai fase stasioner. Kultur bakteri ini selanjutnya diekstraksi menggunakan etil asetat. Ekstrak kasar supernatan bakteri endofit A.conyzoides yang diperoleh kemudian diujikan aktivitas anti quorum sensing menggunakan bakteri Chromobacterium violaceum CV026. Konsentrasi ekstrak kasar supernatan bakteri endofit yang diberikan adalah 2.5 mg ml-1, 3 mg ml-1, dan 3.5 mg ml-1. Hasil penelitian menunjukkan ketiga ekstrak kasar supernatan bakteri endofit A.conyzoides berpengaruh terhadap pengurangan produksi violacein. Ekstrak yang paling tinggi menunjukkan potensi anti quorum sensing adalah ekstrak kasar supernatan bakteri Kurthia (I14) pada konsentrasi 3.5 mg ml-1. Hal tersebut mengindikasikan bahwa ekstrak kasar supernatan A.conyzoides memiliki kemampuan sebagai anti quorum sensing. Aktivitas anti quorum sensing bergantung pada konsentrasi ekstrak kasar supernatan bakteri endofit A. conyzoides yang diberikan pada kultur C.violaceum CV026. The research of potential anti quorum sensing by crude extract of Ageratum conyzoides endophytic bacteria was conducted. Quorum sensing is cell-to-cell communication in bacteria that enable to regulate bacterial physiological function such as biofilm, virulence, and swarming. There are three endophytic bacteria of A. conyzoides have been isolated. There are Pseudomonas, Brochothrix and Kurthia. The purpose of this research was to know potency of anti quorum sensing activity from crude extract of A. conyzoides endophytic bacteria. Three isolates of A. conyzoides endophytic bacteria were inoculated in King's B medium until stationary phase. Furthermore, these bacteria were extracted using etyl acetat. The crude extract of A. conyzoides endophytic bacteria as anti quorum sensing was monitored using Chromobacterim violaceum CV026. The concentration of crude extract of A. conyzoides endophytic bacteria that used in this research were 2.5 mg ml-1, 3 mg ml-1, and 3.5 mg ml-1. The result shown that three crude extracts of A. conyzoides endophytic bacteria can reduce violacein production. The most potential extract that have anti quorum sensing activity is Kurthia at concentration 3.5 mg ml-1. It was indicating quorum sensing inhibition by crude extract A. conyzoides endophytic bacteria. Anti quorum sensing activity was depend on concentration of crude extract A. conyzoides endophytic bacteria that used in C.violaceum CV026 culture.