ISOLASI DAN IDENTIFIKASI FUNGI YANG BERPOTENSI SEBAGAI REMEDIATOR TANAH TERCEMAR OLEH OLI BEKAS KENDARAAN BERMOTOR

• Main Author: Indri Berliani, - (Author)
• Format: Book
• Published: 2019-08-22.
• Subjects: Thesis; NonPeerReviewed
• Online Access: Link Metadata
• Notes: http://repository.upi.edu/39448/1/S_BIO_1500864_Title.pdf; http://repository.upi.edu/39448/2/S_BIO_1500864_Chapter1.pdf; http://repository.upi.edu/39448/3/S_BIO_1500864_Chapter2.pdf; http://repository.upi.edu/39448/4/S_BIO_1500864_Chapter3.pdf; http://repository.upi.edu/39448/5/S_BIO_1500864_Chapter4.pdf; http://repository.upi.edu/39448/6/S_BIO_1500864_Chapter5.pdf; http://repository.upi.edu/39448/7/S_BIO_1500864_Appendix.pdf
• Keywords: QK Botany

ABSTRAK Pencemaran minyak bumi di lahan dan perairan menimbulkan masalah serius bagi lingkungan sekitarnya. Pencemaran minyak bumi dapat berasal dari proses penambangan di darat dan lepas pantai, limbah hasil pengolahan, kecelakan kapal pengangkut minyak bumi, atau dari sisa pencucian minyak dari kapal dan pencemaran oleh oli bekas kendaraan bermotor. Penelitian ini bertujuan untuk mengidentifikasi mikroba dari kelompok fungi yang berpotensi mendegradasi hidrokarbon dari lahan tercemar oleh oli bekas kendaraan bermotor. Penelitian yang telah dilakukan adalah isolasi, seleksi, dan identifikasi fungi potensial pendegradasi hidrokarbon pada tanah tercemar oli bekas kendaraan bermotor di daerah Singaparna, Tasikmalaya. Fungi dikembangbiakan secara bertahap dalam media Potato Dextrose Agar (PDA) yang telah dicampur dengan choramphenicol dan selanjutnya menggunakan media Stone Mineral Salt Solution (SMSS) yang ditambahkan 1% oli bekas kendaraan bermotor. Hasil isolasi diperoleh 4 isolat fungi yang tumbuh pada media Potato Dextrose Agar (PDA). Ciri-ciri setiap isolat dibandingkan berdasarkan kunci determinasi pada Introductory Mycology Fourth Edition, Moulds Isolation, Cultivation, Identification, Smith's Introduction to Industrial Mycology Seventh Edition. Isolat A merupakan genus Penicillium, isolat B merupakan genus Aspergilus, isolat C merupakan genus Trichoderma dan isolat D merupakan genus Mucor. Seleksi pertumbuhan dan kemampuan isolat dalam mendegradasi hidrokarbon dilakukan dengan inokulasi isolat dalam media Stone Mineral Salt Solution (SMSS) yang ditambahkan 1% oli bekas kendaraan bermotor. Dari 4 isolat fungi hanya 3 yang menunjukkan kemampuan pendegradasi hidrokarbon ditandai dengan tumbuh dalam media yang mengandung oli bekas kendaraan bermotor, 3 isolat yang sudah teridentifikasi yaitu Penicillium (Kode isolat A), Aspergilus (Kode isolat B), dan Trichoderma (Kode isolat C). Kata kunci: Bioremediasi, Hidrokarbon, Penicillium, Aspergilus, Trichoderma. ABSTRACT Petroleum pollution in the land and waters creates serious problems for the surrounding environment. Petroleum pollution can originate from onshore and offshore mining processes, processing waste, oil vessel transport accidents, or from oil leaching residues from ships. This study aims to obtain superior indigenic microbes from a group of fungi that have the potential to degrade hydrocarbons from land polluted by used motor vehicle oil. The research that has been carried out is the isolation, selection, and identification of potential fungi for hydrocarbon degradation in motor oil-contaminated soils in Singaparna, Tasikmalaya. The fungus is propagated gradually in the media of Potato Dextrose Agar (PDA) which has been mixed with choramphenicol and subsequently using Stone Mineral Salt Solution (SMSS) media which is added by 1% of used motor vehicle oil. The results of isolation obtained 4 fungi isolates that grow on Potato Dextrose Agar (PDA) media. The characteristics of each isolate were compared based on the key determination in Introductory Mycology Fourth Edition, Molds Isolation, Cultivation, Identification, Smith's Introduction to Industrial Mycology Seventh Edition. Isolate A is the genus Penicillium, isolate B is the genus Aspergilus, isolate C is the genus Trichoderma and isolate D is the genus Mucor. Growth selection and the ability of isolates to degrade hydrocarbons were carried out by inoculating isolates in the Stone Mineral Salt Solution (SMSS) medium added 1% of used motor vehicle oil. From 4 fungi isolates, only 3 showed hydrocarbon degradation ability, 3 isolates were identified namely Penicillium (Code isolate A), Aspergilus (Code isolate B), and Trichoderma (Code isolate C). Three fungi isolates can grow well in media containing used motor vehicle oil. The results of the mushroom growth curve in the three isolates did not experience the lag phase (adaptation phase) because the previous medium provided the same environmental conditions. Keywords: Bioremediation, Hydrocarbons, Penicillium, Aspergillus, Trichoderma.