TOKSISITAS NANOPARTIKEL PERAK ENTOMOPATOGEN TERHADAP LARVA INSTAR TIGA NYAMUK Aedes aegypti

• Main Author: Yasri Ariyanti Maulida, - (Author)
• Format: Book
• Published: 2021-08-13.
• Subjects: Thesis; NonPeerReviewed
• Online Access: Link Metadata
• Notes: http://repository.upi.edu/64866/1/S_BIO_1700838_Title.pdf; http://repository.upi.edu/64866/2/S_BIO_1700838_Chapter%201.pdf; http://repository.upi.edu/64866/3/S_BIO_1700838_Chapter%202.pdf; http://repository.upi.edu/64866/4/S_BIO_1700838_Chapter%203.pdf; http://repository.upi.edu/64866/5/S_BIO_1700838_Chapter%204.pdf; http://repository.upi.edu/64866/6/S_BIO_1700838_Chapter%205.pdf; http://repository.upi.edu/64866/7/S_BIO_1700838_Appendix.pdf
• Keywords: L Education (General); QH301 Biology

Demam Berdarah Dengue (DBD) merupakan penyakit yang disebabkan oleh Nyamuk Aedes aegypti, seiring berkembangnya ilmu pengetahuan dan teknologi, terkait insektisida ramah lingkungan pengendalian nyamuk Ae. aegypti dapat dilakukan dengan memanfaatkan Nanopartikel Perak (NPP) yaitu partikel berukuran 1-100 nm yang didapatkan melalui metode biologis yaitu dengan menggunakan mikroorganisme entomopatogen. Tujuan dari penelitian ini yaitu memberikan informasi mengenai toksisitas nanopartikel perak yang disintesis menggunakan bakteri Bacillus thuringiensis terhadap kematian 50% larva instar tiga Ae. aegypti. Penelitian dilakukan dengan menggunakan uji toksisitas akut. Pada uji pendahuluan (range finding test) 10 larva instar III Ae. aegypti dimasukan kedalam botol vial 10 ml yang berisikan konsentrasi NPP-B. thuringiensis 5 ppm, 10 ppm, 15 ppm, 20 ppm, dan 25 ppm, kemudian presentase mortalitas larva ditentukan, dilanjutkan dengan uji utama (definitive test) dengan konsentrasi NPP-B. thuringiensis 8 ppm, 10 ppm, 12 ppm, dan 14 ppm. Masing-masing uji diamati selama 72 jam. Gejala kematian pada instar III Ae. aegypti diamati menggunakan mikroskop. Hasil pengamatan menggunakan analisa probit menunjukan bahwa uji toksisitas yang dilakukan menghasilkan nilai LC50-72 jam 9,12 ppm. Efek toksisitas NPP-B. thuringiensis menyebabkan banyak bagian tubuh larva rusak dan hancur. Simpulan dari penelitian ini yaitu NPP-B. thuringiensis memiliki nilai toksisitas LC50-72 jam 9,12 ppm < 30 ppm terhadap larva instar III Ae. Aegypti yang dapat dikatakan bersifat sangat toksik. Dengue Hemorrhagic Fever (DHF) is a disease caused by the Aedes aegypti mosquito, along with the development of science and technology, regarding environmentally friendly insecticides the control of Ae. aegypti can be done by utilizing silver nanoparticles that are particles measuring 1-100 nm. Silver Nanoparticles (NPP) are obtained through biological methods by using entomopathogenic microorganisms. The purpose of this study was to provide information on the toxicity of silver nanoparticles synthesized using Bacillus thuringiensis to the mortality of 50% of the third instar larvae of Ae. Aegypti. The study was conducted using an acute toxicity test. In the preliminary test (range finding test) 10 third instar larvae of Ae. aegypti was put into a 10 ml vials bottle containing the concentration of NPP-B. thuringiensis 5 ppm, 10 ppm, 15 ppm, 20 ppm, and 25 ppm, then the percentage of larval mortality was determined, the test was continued with the main test (definitive test) with concentrations of NPP-B. thuringiensis 8 ppm, 10 ppm, 12 ppm, and 14 ppm. Each test was observed for 72 hours. Symptoms of death in the third instar Ae. aegypti were observed using a microscope. Probit analysis showed that the toxicity test carried out resulted in an LC50-72 hours = 9.12 ppm. The toxic effect of NPP-B. thuringiensis causes many parts of the larvae body to be damaged and destroyed. The conclusion of this study is NPP-B. thuringiensis has an LC50-72 hours toxicity value of 9.12 ppm < 30 ppm against third instar larvae of Ae. Aegypti which can be said to be very toxic.