NANOENKAPSULASI L-DOPA DALAM PATI NANOPARTIKEL UNTUK KANDIDAT OBAT ANTI PARKINSON

• Main Author: Dhea Salsabila, - (Author)
• Format: Book
• Published: 2022-08-26.
• Subjects: Thesis; NonPeerReviewed
• Online Access: Link Metadata
• Notes: http://repository.upi.edu/81016/1/S_KIM_1805495_Title.pdf; http://repository.upi.edu/81016/2/S_KIM_1805495_Chapter1.pdf; http://repository.upi.edu/81016/3/S_KIM_1805495_Chapter2.pdf; http://repository.upi.edu/81016/4/S_KIM_1805495_Chapter3.pdf; http://repository.upi.edu/81016/5/S_KIM_1805495_Chapter4.pdf; http://repository.upi.edu/81016/6/S_KIM_1805495_Chapter5.pdf; http://repository.upi.edu/81016/7/S_KIM_1805495_Appendix.pdf
• Keywords: Q Science (General); QD Chemistry

Penyakit Parkinson merupakan penyakit neurodegeneratif yang disebabkan oleh rusaknya saraf penghasil dopamin di otak. L-DOPA yang merupakan prekursor dopamin merupakan obat yang digunakan untuk menangani penyakit Parkinson. Proses nanoenkapsulasi L-DOPA menggunakan pati nanopartikel bertujuan untuk meningkatkan efisiensi drug-delivery sehingga meningkatkan konsentrasi L-DOPA yang dapat rilis di otak. Penelitian ini bertujuan untuk memperoleh kondisi optimum proses nanoenkapsulasi L-DOPA dalam pati nanopartikel, dan mengetahui karakteristik, efisiensi enkapsulasi serta kemampuan drug-release produk nanoenkapsulasi. Proses nanoenkapsulasi dilakukan dengan metode difusi pelarut termodifikasi pada beberapa variasi perbandingan massa. Karakterisasi produk nanoenkapsulasi menggunakan Spektroskopi Fourier Transformation Infrared (FTIR), Scanning Electron Microscopy (SEM), dan Transmission Electron Microscopy (TEM). Uji efisiensi enkapsulasi diukur melalui perubahan konsentrasi L-DOPA menggunakan spektrometer UV-Vis. Uji drug release dilakukan menggunakan kantung dialisis yang diukur pada pH 1,2 dan 7,4 selama 5 jam. Berdasarkan penelitian yang dilakukan, kondisi optimum nanoenkapsulasi L-DOPA dalam pati nanopartikel diperoleh pada perbandingan massa pati nanopartikel, maltodekstrin dan L-DOPA sebesar 1 : 3 : 1. Hasil karakterisasi SEM menunjukkan bahwa produk nanoenkapsulasi mempunyai morfologi berupa sferis. Hasil karakterisasi TEM menunjukkan ukuran partikel produk nanoenkapsulasi berkisar 17,77-49,43 nm. Hasil karakterisasi FTIR menunjukkan adanya puncak baru pada bilangan gelombang 1653 cm-1 dari C=O stretch,dan 1570 cm-1 dari N-H bend. Tingkat efisiensi enkapsulasi tertinggi didapatkan sebesar 46,11%. Kemampuan drug release diperoleh sebesar 40,93% untuk pH 1,2 dan 48,28% untuk pH 7,4 pada 5 jam pengujian. Parkinson's disease is a neurodegenerative disease caused by damage to the dopamine-producing nerves in the brain. L-DOPA, which is a precursor to dopamine, is a drug used to treat Parkinson's disease. The L-DOPA nanoencapsulation process using starch nanoparticles aims to increase drug-delivery efficiency so as to increase the concentration of L-DOPA that can be released in the brain. This study aims to obtain the optimum conditions for the L-DOPA nanoencapsulation process in starch nanoparticles, and to determine the characteristics, encapsulation efficiency and drug-release ability of nanoencapsulated products. The nanoencapsulation process was carried out using a modified solvent diffusion method in several variations of mass ratios. Characterization of nanoencapsulated products using Fourier Transformation Infrared Spectroscopy (FTIR), Scanning Electron Microscopy (SEM), and Transmission Electron Microscopy (TEM). Encapsulation efficiency test was measured by changing the concentration of L-DOPA using a UV-Vis spectrometer. The drug release test was carried out using a dialysis bag measured at pH 1.2 and 7.4 for 5 hours. Based on the research conducted, the optimum conditions for L-DOPA nanoencapsulation in starch nanoparticles were obtained at a mass ratio of starch nanoparticles, maltodextrin and L-DOPA of 1: 3: 1. The results of SEM characterization showed that the nanoencapsulated product had a spherical morphology. The results of TEM characterization showed that the particle size of the nanoencapsulated product ranged from 17.77 to 49.43 nm. The results of FTIR characterization show that there are new peaks at wave number 1653 cm-1 from C=O stretch, and 1570 cm-1 from N-H bend. The highest level of encapsulation efficiency was obtained at 46.11%. The drug release ability was obtained at 40.93% for pH 1.2 and 48.28% for pH 7.4 at 5 hours of testing.