EFEKTIVITAS ISOLAT Actinomycetes SEBAGAIANTIFUNGI TERHADAP PERTUMBUHAN Candida albicans SECARA IN VITRO
Actinomycetes are Gram-positive and anaerobic bacteria noted for its rodshaped forms. Actinomycetes also has an antibiotic and antifungal activity. Candida albicans is the one kind of fungus that infects simultaneously with HIV/AIDS suffers. This study aims to identify the effectiveness of Actinomyc...
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2019-10-22.
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| 520 | |a Actinomycetes are Gram-positive and anaerobic bacteria noted for its rodshaped forms. Actinomycetes also has an antibiotic and antifungal activity. Candida albicans is the one kind of fungus that infects simultaneously with HIV/AIDS suffers. This study aims to identify the effectiveness of Actinomycetes isolate as the antifungal toward C. albicans growth by in vitro. Soil samples were taken at Kebun Raya Bogor after considering their temperature, pH, and soil humidity. Collected samples were planted on Starch Casein Agar (SCA) through pour plate method with three dilution series of 10 -4 , 10 -5 ,10 -6 . Actinomycetes isolates found in the samples were 15 isolates. The media used to see the obstacles zone of C. albicans was Sabouraud Dextrose Agar (SDA) with well method diffusion. From the three-dilution series, the study gets the size average of clear zone 3,78 mm, 4,03 mm and 3,52 mm. One way Anova (α 0,05) result also confirmed the difference of obstacles zone levels between C. albicans concentration. The compound comes from Actinomycetes that has potential as antifungal with one of its mechanism's bonds ergosterol and resists fungi protein synthesis. | ||
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