TOXICITY TEST OF HUMAN CD34+ STEM CELLS IN SPRAGUE DAWLEY RATS (PRELIMINARY STUDY)
Toxicity of CD34+ allogenic stem cell therapy in cartilage defect remains to be evaluated. We investigated the toxic reaction of CD34+ stem cells intervention in non engineered Sprague Dawley (SD) rats. Three male SD Rats were divided into 3 treatment; treatment 1 received intraarticular injection o...
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| Format: | Book |
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Faculty of Agriculture Universitas Sebelas Maret,
2017-02.
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| Summary: | Toxicity of CD34+ allogenic stem cell therapy in cartilage defect remains to be evaluated. We investigated the toxic reaction of CD34+ stem cells intervention in non engineered Sprague Dawley (SD) rats. Three male SD Rats were divided into 3 treatment; treatment 1 received intraarticular injection of CD34+ cells (105 cells) from human peripheral blood, treatment 2 received intraarticular injection of CD34+ cells (105 cells) human cord blood combined with hyaluronic acid and growth factors, and treatment 3 received intraarticular injection of CD34+ cells (106 cells) human cord blood combined with hyaluronic acid and growth factors. All rats were performed hematologic examination, blood chemistry examination, liver histopathology examination, and kidney histopathology examination. All rats showed good condition. There are non significant increased in hematologic and blood chemistry examination in all treatment. The liver and kidney histopathology examination showed normal condition. There is no hiperacute (edema, and systemic bleeding) and no acute rejection. In conclusion, human CD34+ cells did not induced toxicity reaction on SD rats. |
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| Item Description: | http://repository.upnvj.ac.id/370/1/artikel_prosiding_toxicity_test_of_human_cd34.pdf |